Figure 2

Evolutionary history of eukaryotic porin sequences – stramenopiles and plants. The phylogenetic tree, continued in Figure 3, is based on NJ and Bayesian analysis of 141 VDAC sequences. Stramenopile sequences were used as the out group. Levels of confidence of the nodes are only provided if support is above 66%. The numbers are based on posterior probability values generated by Bayesian analysis and on bootstrap analysis in combination with NJ analysis (italics). The presence of the GLK (G) and Eukaryotic porin motifs (E) are indicated towards the right of the phylogenetic tree. Note among the plants the GLK domain appears as the STK (S) motif; an X indicates the absence of the motif. Lower case g designates a G-any-K or G-any-R, where "any" refers to any other amino acid. The lower case s indicates S-any-K or S-any-R. The minus (-) indicates that the sequence was incomplete and thus the GLK and eukaryotic porin motifs could not be identified. Nodes designated by a number (1–10) are discussed within the text. Underlined accession numbers are those of VDACs that do not contain the signature motif identified in this study.