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Table 3 Gene-specific oligonucleotides for cloning and expression analyses of dog CEA family members

From: Species-specific evolution of immune receptor tyrosine based activation motif-containing CEACAM1-related immune receptors in the dog

gene oligonucleotide name oligonucleotide sequence location of primers (exon) size of PCR product (bp)*
CEACAM1 cfCEACAM1-5i-f
cfCEACAM1-3i-r
5'-CGGCAGCTGCTCTCACC
5'-CCAGCAGGACAGGTTGCAT
5'-UTR
3'-UTR
1624
  CEACAM1cf-5i
CEACAM1cf-3i
5'-GTCATGAAGCTTCGGCAGCTGCTCTCACC
5'-GTCAGTTCTAGACCAGCAGGACAGGTTGCAT
5'-UTR
3'-UTR
1648
  cfCEACAM1TM-f
cfCEACAM1C3-r
5'-AGTTCTGGCCTTCCACCTG
5'-GGATGAGGAGGCTGAAGTTG
TM domain
Cyt 3
270
  cfCEACAM1N-f
cfCEACAM1C3-r
5'-CATCACCCTGAACGACACTG
5'-GGATGAGGAGGCTGAAGTTG
N domain
Cyt 3
1183
CEACAM23 cfaCC23-N-fo
cfaCC23-T-re
5'-TCCAGAACATCACCCTGAGC
5'-GACACCCCAGGGAAGCTG
N domain
TM domain
1008
  cfCEACAM1-5i-f
cfaCC23-3-re
5'-CGGCAGCTGCTCTCACC
5'-GAGCAGTACCATGGGGAAAC
5'-UTR
3'-UTR
1616
CEACAM24 cfaCC24-N-fo
cfaCC24-T-re
5'-GTAGTAGACACACGAGTAATTGT
5'-CACAAGCGCTACTCCAATCA
N domain
TM domain
258
  cfCEACAM1-5i-f
cfaCC24-3-re
5'-CGGCAGCTGCTCTCACC
5'-GAGGGTTCCAGGGTGAAACT
5'-UTR
3'-UTR
1069
CEACAM25 cfaCC25-N-fo
cfaCC25-T-re
5'-AATCTGCCTGGGGATCTTCT
5'-AATACGCCAGGGTAGCCATG
N domain
TM domain
352
  cfCEACAM1-5i-f
cfaCC25-3-re
5'-CGGCAGCTGCTCTCACC
5'-GGAAGGAGGGTTCCAAGGT
5'-UTR
3'-UTR
807
CEACAM28 (CEACAM30)** cfaCC28-N-fo
cfaCC28-T-re
5'-TTTGAAACTGCACTAATACGG
5'-TTGTGTGGAGCAGGAGACA
N domain
TM domain
703 (427)
  cfCEACAM1-5i-f
cfaCC28-3-re
5'-CGGCAGCTGCTCTCACC
5'-GGAAGGAGAAGTGTCATGAGGT
5'-UTR
3'-UTR
1374 (1095)
CEACAM29 cfaCC29-N-fo
cfaCC29-T-re
5'-ACATCACCGTGAATAATGCC
5'-CGAGTGCTAGTTCGAGCAGA
N domain
TM domain
447
  cfCEACAM1-5i-f
cfaCC29-3-re
5'-CGGCAGCTGCTCTCACC
5'-CAGAAGGTTACAGGGGCTCA
5'-UTR
3'-UTR
844
  1. * Size of largest cloned or predicted (CEACAM29) splice variant is shown
  2. ** CEACAM30 cDNA were also detected using the CEACAM28 primers (size of PCR products in brackets)
  3. Cyt, cytoplasmic domain; TM, transmembrane domain; UTR, untranslated region