Table 3 Gene-specific oligonucleotides for cloning and expression analyses of dog CEA family members
gene | oligonucleotide name | oligonucleotide sequence | location of primers (exon) | size of PCR product (bp)* |
|---|---|---|---|---|
CEACAM1 | cfCEACAM1-5i-f cfCEACAM1-3i-r | 5'-CGGCAGCTGCTCTCACC 5'-CCAGCAGGACAGGTTGCAT | 5'-UTR 3'-UTR | 1624 |
| Â | CEACAM1cf-5i CEACAM1cf-3i | 5'-GTCATGAAGCTTCGGCAGCTGCTCTCACC 5'-GTCAGTTCTAGACCAGCAGGACAGGTTGCAT | 5'-UTR 3'-UTR | 1648 |
| Â | cfCEACAM1TM-f cfCEACAM1C3-r | 5'-AGTTCTGGCCTTCCACCTG 5'-GGATGAGGAGGCTGAAGTTG | TM domain Cyt 3 | 270 |
| Â | cfCEACAM1N-f cfCEACAM1C3-r | 5'-CATCACCCTGAACGACACTG 5'-GGATGAGGAGGCTGAAGTTG | N domain Cyt 3 | 1183 |
CEACAM23 | cfaCC23-N-fo cfaCC23-T-re | 5'-TCCAGAACATCACCCTGAGC 5'-GACACCCCAGGGAAGCTG | N domain TM domain | 1008 |
| Â | cfCEACAM1-5i-f cfaCC23-3-re | 5'-CGGCAGCTGCTCTCACC 5'-GAGCAGTACCATGGGGAAAC | 5'-UTR 3'-UTR | 1616 |
CEACAM24 | cfaCC24-N-fo cfaCC24-T-re | 5'-GTAGTAGACACACGAGTAATTGT 5'-CACAAGCGCTACTCCAATCA | N domain TM domain | 258 |
| Â | cfCEACAM1-5i-f cfaCC24-3-re | 5'-CGGCAGCTGCTCTCACC 5'-GAGGGTTCCAGGGTGAAACT | 5'-UTR 3'-UTR | 1069 |
CEACAM25 | cfaCC25-N-fo cfaCC25-T-re | 5'-AATCTGCCTGGGGATCTTCT 5'-AATACGCCAGGGTAGCCATG | N domain TM domain | 352 |
| Â | cfCEACAM1-5i-f cfaCC25-3-re | 5'-CGGCAGCTGCTCTCACC 5'-GGAAGGAGGGTTCCAAGGT | 5'-UTR 3'-UTR | 807 |
CEACAM28 (CEACAM30)** | cfaCC28-N-fo cfaCC28-T-re | 5'-TTTGAAACTGCACTAATACGG 5'-TTGTGTGGAGCAGGAGACA | N domain TM domain | 703 (427) |
| Â | cfCEACAM1-5i-f cfaCC28-3-re | 5'-CGGCAGCTGCTCTCACC 5'-GGAAGGAGAAGTGTCATGAGGT | 5'-UTR 3'-UTR | 1374 (1095) |
CEACAM29 | cfaCC29-N-fo cfaCC29-T-re | 5'-ACATCACCGTGAATAATGCC 5'-CGAGTGCTAGTTCGAGCAGA | N domain TM domain | 447 |
| Â | cfCEACAM1-5i-f cfaCC29-3-re | 5'-CGGCAGCTGCTCTCACC 5'-CAGAAGGTTACAGGGGCTCA | 5'-UTR 3'-UTR | 844 |