Figure 1

Characterisation of Orly transcripts. A: Structure of Orly as a chimera of relict partial Ssty1, Asty and Sly loci (not to scale). Arrows indicate orientation of the relict loci. Exons are coloured according to their origin. B: Structure of Orly transcripts found in the nr database. C: Primer locations used to characterise Orly splice variants (sequences given in Table 1) D: Structure of variant transcripts detected in the screen. The primer pairs which amplify each variant form are indicated. Exons 3a and 3b are short forms of Ssty1- derived exon S3, and are variably included in Orly transcripts. Of note is that the majority of splice variant forms do not conform to the splicing patterns of the known transcripts shown in panel A. + indicates "forward" transcripts - indicates the "reverse" transcript detected with primer pair N1.f/N2.r2