Phylogeography of the Solanaceae-infecting Basidiomycota fungus Rhizoctonia solani AG-3 based on sequence analysis of two nuclear DNA loci

Table 6 Primers for PCR amplification and sequencing reactions of two nuclear DNA fragments from Rhizoctonia solani AG-3 PT and TB

Loci	Product size	Oligo	Length	Tm (°C)	GC%	Sequence (5' to 3')
pP42F ^a	401	F119L	19	60.4	57.9	GTTGGATCACGTCGCTCAG
		F518R	20	59.7	55.0	TAGTATGGGATACCCACGCC
pP89^b	1090	F02	21	62.3	52.4	TTTGAGGAAGAACGCGTACGC
		R10	21	60.7	42.9	TGTCATTGAAAATACGGCCGA
	Internal	L253	22	59.9	50.0	GTGTGTACTTGTTGGGGAGACA
	primers	R790	22	59.7	45.5	GGTCGTGGGCAAATCTTAATAC

^a Similar to lsc2p gene encoding for beta subunit of succinyl-CoA ligase (synthetase; ATP-forming; a mitochondrial enzyme of the TCA cycle) localized at nuclear chromosome VII from Saccharomyces cerevisiae, accession 6321683 deposited at GenBank (BLASTX2.2.3 score of 89.0 and E-value of 9e-³¹) or succinyl-CoA ligase [GDP-forming] beta-chain, hydrogenosomal precursor (succinyl-CoA synthetase, beta chain) from Neocallimastix frontalis, accession 9789854 (BLASTX2.2.3 score of 99 and E-value of 3e^-33)
^b Similar to crm1 gene, encoding for chromosome region maintenance protein 1 or exportin 1 from Schizosaccharomyces pombe, accession 19856107 (BLASTX2.2.3 score of 291 and E-value of e^-101)

ISSN: 2730-7182