Figure 2

Phylogenetic comparison of Juan sequences and their hosts. A. Consensus tree of host phylogeny constructed with MrBayes (see methods) using nucleotide sequences of Vg-C, a single copy gene. Vg-C from An. gambiae is used to root the tree. Clade credibility values from 150,000 generations are given at each node. B. Consensus tree constructed with MrBayes using conceptually translated sequences of Juan from PCR and genomic database (Aa, Ae. aegypti, Ag, An. gambiae). Clade credibility values from 200,000 generations are given at each node or beside brackets. Ag-Jen-4 and other An. gambiae sequences correspond to families previously identified (Biedler and Tu 2003). Jockey elements from D. melanogaster (Dm) and C. tentans (Ct) are used to root the tree. Accessions are given beside sequence names. An asterisk indicates that the reading frame was intact. A bold capital "L" indicates that the sequence was obtained from a genomic library. The first three letters of a species name is used to label PCR and library sequences. Genus names are in bold beside brackets. Juan-A is from Ae. aegypti and Juan-C is from C. pipiens. Abbreviations: Ae. aegypti (aeg); Ae. albopictus (alb); Ae. simpsoni (sim); Ae. polynesiensis (pol); Ae. vexans (vex); C. molestus (mol); C. quinquefasciatus (qui); C. restuans (res); C. tarsalis (tar); D. cancer (can); O. atropalpus (atr); O. bahamensis (bah); O. epactius (epa); O. taeniorhinchus (tae); O. triseriatus (tri); P. ciliata (cil); W. michelli (mic). Number indicates clone from PCR. Ae. aegypti and An. gambiae sequences from genomic database (e.g. Ag-Jock-1, AaJockeyEle2) can be found in the TEfam database [57]. See additional files 1 and 2 for alignments used for phylogenetic inference.