Figure 2

Phylogeny of the Cyanidiales inferred from a maximum likelihood (ML) analysis using the site-specific GTR evolutionary model and rbc L sequences. Results of the ML-all bootstrap analysis are shown above the branches on the left of the slash mark, whereas the results of the bootstrap analysis of ML-1^st + 2^nd data set are shown on the right of the slash mark. The results of the protein maximum likelihood bootstrap analysis using the JTT + Γ model are shown below the branches. Only bootstrap values ≥ 50% are shown. The thick nodes represent ≥ 95% Bayesian posterior probability for clades using the site-specific GTR model. The sequences arising from a previous study [6] are marked with asterisks. The percentage values shown for each sampling site indicate the proportion of Cyanidiales members that were recovered in the different PCR-based environmental libraries. About 20 clones were sampled from each site (for the actual number, see Table 1).