Figure 4

Sequences upstream of rps14 consistent with separate transfers in Cyperaceae and Poaceae. Shown is an amino acid alignment of the 5' end of RPS14 and upstream sequences. Sequences are aligned relative to Oryza nuclear rps14 with identical amino acids indicated by dots, gaps by dashes, missing data by "~", and intron-exon junction by a triangle. The lack of homology between the putative targeting peptides (the N-terminal extensions past the methionine start of the mitochondrial rps14 genes) of the Carex and grass nuclear genes is consistent with their being the result of separate functional transfers. Except for a methionine so close (7 amino acids) to the start of the mature RPS14 protein as to produce a potential targeting peptide too short to likely be functional, the Carex gene lacks a potential (methionine) start codon in the region for which sequence has been obtained.