Figure 2

Protein sequence relationships of the C-terminal regions of fish and tetrapod TSPs. A, Sequences corresponding to aa 674–1152 of human TSP-1 were aligned in CLUSTALW. The unrooted tree was prepared in Phylip DRAWTREE and is presented in Phylodendron. The cluster of tetrapod TSP-5 sequences within the broad TSP-4/TSP-5 grouping is arrowed. B, The same sequences were aligned in TCOFFEE and analyzed by the maximum-likelihood method, PHYML, including 100 bootstrap cycles. Bootstrap values are given for the major internal nodes: values above 70 are taken to indicate stability of the branchpoint. Scale bars = 0.1 substitutions/site. Key : Dr = Danio rerio; Gg = Gallus gallus; Hs = Homo sapiens; Mm = Mus musculus; Tn = Tetraodon nigriviridis; Tr = Takifugu rubripes; Xt = Xenopus tropicalis.