2,7
|
C427Y
|
null
|
null
|
all HMTases
|
zinc coordination [37]
|
21
|
E455V
|
Hypo-morph
|
null
|
all HMTases
|
-
|
31
|
E455K
| | |
all HMTases
|
intramolecular charge interaction [33]
|
3
|
R469C
| |
null
|
all HMTases
|
intramolecular charge interaction [33]
|
27
|
G491R
| |
null
|
all HMTases
|
Adomet (substrate) binding [32]
|
12,23
|
V492E
| | |
all HMTases
|
intramolecular hydrophobic interaction [33]
|
4,5
|
G509E
|
null
|
null
|
all HMTases
|
structural residue glycine [33]
|
Class II – compensable deviated residues (CPDs)
|
30
|
A437T
| |
null
|
Su(var)3-9, dim5
|
T in all beetles, compensated by L435G/S
|
9
|
C505Y
| |
null
|
Su(var)3-9, G9a, SUVH, SETDB
|
L in Araneus diadematus, compensated by V585A
|
34
|
E506K
| | |
Su(var)3-9, G9a, SUVH
|
K in Acyrthosiphon pisum, compensation unknown (many substitutions in contact area of the residue)
|
320
|
D536N
| | |
Su(var)3-9, dim5, G9a
|
N in Dictyostelium discoideum, compensated by Y537G and N538D
|
32
|
S562F
|
null
|
null
|
all HMTases
|
L in some G9a proteins, compensated by S599G
|
13
|
P571S
|
Hypo-morph
|
Hypo-morph
|
Su(var)3-9 (Drosophila), SUVH, dim5
|
S in Mus musculus Suv39h2 and in mosquitos, compensated by C572V and H583R; A ribose hydroxyl of the methyltransferase reaction end product AdoHcy interact with the side chain carboxyl of the homologous D202 in Neurospora dim-5 [32]
|
10
|
D601N
|
null
|
Hypo-morph
|
most of all HMTases
|
N in mosquitos, Acyrthosiphon pisum and in some other HMTases, compensation ambiguous; involved in formation of lysine (substrate) binding channel [32]
|
8
|
N607Y
|
null
|
Hypo-morph
|
Su(var)3-9 (Drosophila)
|
no substitution or residue not conserved
|
25
|
P611L
|
Hypo-morph
|
Hypo-morph
|
Su(var)3-9 (Drosophila)
|
no substitution or residue not conserved
|
319
|
S616L
| | |
Su(var)3-9 (Drosophila)
|
no substitution or residue not conserved
|
22
|
L634F
|
Hypo-morph
|
Hypo-morph
|
Su(var)3-9, dim5, SETDB
|
F in Enallagma cyathigerum, compensated by F635M
|
Class III – Uncompensated gain of function alleles
|
ptn
|
R529S
|
Hyper-morph
|
Hyper-morph
|
Su(var)3-9
|
S in three fishes and in Acyrthosiphon pisum, local compensation is excluded; the side chain of the homologous L317 in Neurospora dim-5 is involved in HMTase activity [32]
|