Comparison of selected follistatin-like proteins. FST: follistatin. (a) Multiple alignment of the cysteine-rich domain. Darker shading indicates perfect conservation, lighter shading indicates positions that can be explained assuming one or two mutations. (b) Neighbor-joining tree rooted using human osteonectin/SPARC (NP_003109) as outgroup. Numbers on branches indicate the percent support in 1000 bootstrap replicates. The horizontal bar indicates 10% divergence along each branch. The column on the right indicates the scaffold in which each gene is located (fugu) or its genomic location in Mb coordinates and cytogenetic band (human).