Table 2 Oligonucleotides used for reverse transcription, PCR amplification and sequencing of the Qβ genome.
From: Evolution of phage with chemically ambiguous proteomes
Oligo name | Position on phage genome | Sequence (5' --> 3') |
|---|---|---|
Qb5'2 | 1–40 | GGGGACCCCCTTTAGGGGGTCACCTCACACAGCAGTACTT |
Seq1A | 385–411 | GCGAACCTTGTCATTAATGACTTTCTG |
Seq1revA | 510–486 | CATAGTGCCATACTTGGCATTAAAC |
Seq2A | 826–852 | GCCGTTGCTTACTTTAAACTGAAAGGG |
Qb.rtA3 | 948–928 | GGACACAGGCCGAAGGGAACC |
Seq3A | 1295–1315 | TTTTATTAACCCAACGCGTAA |
Qb.rtA4 | 1427-1401 | ATTTACCCCACGCGGATTGAGGACCAG |
Seq4A | 1730–1752 | GAACCCAGCGTATTGAACACTGC |
Qb.rtA5 | 1948-1922 | ACATCAAATTCGCGAGGCTGGAGTTCA |
AmpB | 1998–2020 | CGGCTTAGTTATACCACGTTCCG |
Qb.rtA | 2190-2171 | CGCTAAGAGAGCAATAAGCA |
Seq1B | 2374–2392 | GTAACTCTCTCAGCGCACAA |
Seq1revB | 2495-2470 | AGAGTTAAATGGCGACTGACCATAGG |
Seq2B | 2820–2841 | GGTGCTACAACAACGAATAACC |
Qb.rtB4 | 2947-2925 | CGAAGATGTGTAAAGCTATGGTC |
Seq3B | 3280–3303 | CTGACGGTATGTGTTGTTACCTACG |
Qb.rtB3 | 3385-3364 | TCACAAACGGAACGAGCGAGAG |
Seq4B | 3738–3760 | ATACCTGATGGTTACGGTGATGG |
Qb.rtB2 | 3852-3828 | GGTCCCTTGTATGGTCCGTAATCAC |
3'Qb-pTlink | 4217-4201 | B-AAAAAAAAAAAAAAAAAATGGGAGGAGAGAGGGCAa |