Figure 6
From: Identification of glutathione (GSH)-independent glyoxalase III from Schizosaccharomyces pombe
The subcellular localization of the endogenous S. pombe DJ - 1 and Hsp31 proteins. Cells expressing different GFP tagged fusion proteins were grown to stationary phase in YES. The subcellular localization of GFP fusion proteins was examined and photographed by fluorescence microscopy. Left panels show the fluorescent signals for GFP-tagged SpDJ-1 (A), Hsp3101-GFP (B) and Hsp3102-GFP (C), middle panels show nuclear localization of a known nuclear protein, tRNA 3′ endonuclease tRNase Z, SpTrz1, tagged with red fluorescent protein (RFP), and right panels show overlaid images of GFP and RFP signals.