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Table 2 ANCOVA of gene copy number effects on relative Hsp70 mRNA abundance

From: Inducing extra copies of the Hsp70 gene in Drosophila melanogaster increases energetic demand

 

Duration of exposure to 36°C

Factor

0 min

15 min

30 min

60 min

Act5C 1

b = 0.67 ± 0.13 ***

b = 0.98 ± 0.07 ***

b = 0.85 ± 0.07 ***

b = 0.64 ± 0.10 ***

Genotype

F = 1.34

F = 22.2 ***

F = 35.4 ***

F = 31.9 ***

Genotype effects2:

    

 6 copy – 3 copy

−0.66

−2.23 **

−1.56 ***

−1.76 ***

 12 copy – 3 copy

−0.99

−1.90 **

−1.66 ***

−1.91 ***

 12 copy – 6 copy

−0.33

+0.33

−0.09

−0.14

  1. 1 Reference gene expression included as a covariate; b is the slope estimate.
  2. 2 Estimates using Tukey’s post-hoc contrasts; smaller values of the Ct indicate higher expression levels.
  3. * P<0.05, ** P<0.01, *** P<0.001.
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BMC Ecology and Evolution

ISSN: 2730-7182

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