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Table 1 PCR primers used in this study

From: Genome differentiation in a species pair of coregonine fishes: an extremely rapid speciation driven by stress-activated retrotransposons mediating extensive ribosomal DNA multiplications

Name Region of DNA/FISH probe Primer sequence (5′to3′) Ref.
28S A 3′ end of the 28S rDNA involving the regions A and B, F primer AAA CTC TGG TGG AGG TCC GT [61]
28S B Internally nested in the regions A and B of the 28S rDNA, R primer CTT ACC AAA AGT GGC CCA CTA [61]
28S C1 5′ end of the 28S rDNA adjacent to the ITS2, F primer ACC CGC TGA ATT TAA GCA T [62]
28S D2 Internally nested in the region C3 involving D2, C2, D1, C1, R primer TCC GTG TTT CAA GAC GGG [63]
ITS1 3′ end of the 18S rDNA adjacent to the ITS1, F primer TCC GTA GGT GAA CCT GCG G [64]
ITS2 3′ end of the 5.8S rDNA adjacent to the ITS2, R primer GCT GCG TTC TTC ATC GAT GC [64]
ITS3 5′ end of the 5.8S rDNA adjacent to the ITS1, F primer GCA TCG ATG AAG AAC GCA GC [64]
ITS4 5′ end of the 28S rDNA adjacent to the ITS2, R primer TCC TCC GCT TAT TGA TAT GC [64]
NS1 5′ end of the 18S rDNA F primer GTA GTC ATA TGC TTG TCT [64]
NS2 18 S rDNA R primer GGC TGC TGG CAC CAG ACT TGC [64]
NS3 18 S rDNA F primer GCA AGT CTG GTG CCA GCA GCC [64]
NS4 18 S rDNA R primer CTT CCG TCA ATT CCT TTA AG [64]
NS5 18 S rDNA F primer AAC TTA AAG GAA TTG ACG GAA G [64]
NS6 18 S rDNA R primer GCA TCA CAG ACC TGT TAT TGC CTC [64]
NS7 18 S rDNA F primer GAG GCA ATA ACA GGT CTG TGA TGC [64]
NS8 3′ end of the 18S rDNA R primer TCC GCA GGT TCA CCT ACG GA [64]
RTX1F1 Rex1 F primer TTC TCC AGT GCC TTC AAC ACC [28]
RTX1R3 Rex1 R primer TCC CTC AGC AGA AAG AGT CTG CTC [28]
RTX3F1 Rex3 F primer TAC GGA GAA AAC CCA TTT CG [65]
RTX3F2 Rex3 F primer AAC ACC TTG GCT GCG CCT AG [65]
RTX3F3 Rex3 F primer CGG TGA YAA AGG GCA GCC CTG [28]
RTX3R1 Rex3 R primer AAA GTT CCT CGG TGG CAA GG [65]
RTX3R2 Rex3 R primer CCR GGG GTG GAT GAR RTC CGC CC [65]
RTX3R3 Rex3 R primer TGG CAG ACN GGG GTG GTG GT [28]
RTX6F Rex6 F primer TAA AGC ATA CAT GGA GCG CCA C [28]
RTX6R Rex6 R primer GGT CCT CTA CCA GAG GCC TGG G [28]