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Figure 1 | BMC Evolutionary Biology

Figure 1

From: Molecular co-evolution of a protease and its substrate elucidated by analysis of the activity of predicted ancestral hatching enzyme

Figure 1

Cross-species digestion of egg envelope by hatching enzymes. (A) Photographs of sections of intact fertilized medaka egg envelope, and those treated with hatching enzymes, HCEs, and LCEs. Arrows indicate the thickness of egg envelope. The medaka egg envelope was swollen either by purified medaka HCE (MHCE) or by purified Fundulus HCE (FHCE1/2). The swollen envelope was solubilized by purified MLCE, but not by purified FLCE. (B) Swelling activity of HCEs towards the medaka egg envelope, and (C) time course of the solubilization of the swollen envelope by LCEs. (D) Photographs of sections of intact fertilized egg envelope from Fundulus and those treated with hatching enzymes, HCEs, and LCEs. Arrows indicate the thickness of the envelope. The Fundulus envelope was also swollen either by MHCE or by FHCE1/2. The swollen envelope was solubilized by FLCE, but not by MLCE. Swelling activity of HCEs towards Fundulus envelope (E), and time course of the solubilization of the swollen envelope by LCEs (F). Scale bars, 100 μm.

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BMC Ecology and Evolution

ISSN: 2730-7182

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