Localisation of the RedCAP protein in complex plastids of diatoms. (A) Bipartite plastid targeting sequences in photosynthetic Chromista. The conserved “ASAFAP”-motif [32, 33] at the interface between signal and transit peptides is marked. (B) Sequence of the Phaeodactylum tricornutum RedCAP full length GFP fusion construct. (C) Expression of the full-length RedCAP:GFP fusion constructs in P. tricornutum. (D) Expression of GFP without targeting pre-sequence in P. tricornutum. Panels show microscopical images of transmitted light (differential interference contrast, DIC), chlorophyll autofluorescence, GFP fluorescence and a merged image from left to right, fluorescence images are maximum intensity projections of 14 slices of a 4.9 μm image stack, scale bars represent 10 μm.