Bayesian species delimitation for Pontohedyle. A. Results assuming our primary species hypothesis guide tree (12 MOTUs). B. Results assuming a guide tree, in which each population is treated as separate species (30 populations – MOTUs are indicated at the left side of the graph). Speciation probability limits are provided for each node under different prior estimates on ancestral population size (θ) and divergence times (τ): 1) prior means 0.1 (blue), 2) prior mean θ = 0.001, τ = 0. 1 (green), 3) prior mean θ = 0.1, τ = 0.001 (red), 4) prior means 0.001 (black). Posterior probabilities are calculated as mean values from repeated analyses. We applied different algorithms and starting seeds, as recommended in the BP&P manual .