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Figure 2 | BMC Evolutionary Biology

Figure 2

From: Proteomic characterization and evolutionary analyses of zona pellucida domain-containing proteins in the egg coat of the cephalochordate, Branchiostoma belcheri

Figure 2

(A-B). The unfertilized (top) and fertilized (bottom) B. belcheri eggs with scale bars indicating the size and elevation of the egg coat before and after fertilization. The arrows indicate the egg coats that are the subjects of this study. (C) and (D) show the protein fractions resolved from the unfertilized (panel C) and the fertilized (panel D) egg coat extracts by the SDS-PAGE gel electrophoresis. The gel slicing schemes used for LC-MS/MS analyses are denoted on the right side of each lane. Approximately 20 μg of egg coat extract were loaded on each lane.

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