| | |
Nucl.
|
Dilution ratio
|
|---|
|
Mixture
|
Rep.
|
position
|
0:100
|
10:90
|
20:80
|
30:70
|
40:60
|
50:50
|
60:40
|
70:30
|
80:20
|
90:10
|
100:0
|
|
A x B
|
1
|
666
|
C
|
C
|
C
|
Y
|
Y
|
Y
|
Y
|
T
|
T
|
T
|
T
|
| |
2
| |
C
|
C
|
C
|
Y
|
Y
|
Y
|
Y
|
T
|
T
|
T
|
T
|
| |
3
| |
C
|
C
|
C
|
Y
|
Y
|
Y
|
Y
|
T
|
T
|
T
|
T
|
|
C x D
|
1
|
366
|
A
|
A
|
R
|
R
|
R
|
R
|
R
|
G
|
G
|
G
|
G
|
| |
2
| |
A
|
A
|
R
|
R
|
R
|
R
|
G
|
G
|
G
|
G
|
G
|
| |
3
| |
A
|
A
|
R
|
R
|
R
|
R
|
G
|
G
|
G
|
G
|
G
|
|
E x F
|
1
|
120
|
T
|
T
|
T
|
W
|
W
|
W
|
W
|
W
|
A
|
A
|
A
|
| |
2
| |
T
|
T
|
T
|
W
|
W
|
W
|
W
|
W
|
A
|
A
|
A
|
| |
3
| |
T
|
T
|
T
|
W
|
W
|
W
|
W
|
W
|
A
|
A
|
A
|
| |
1
|
363
|
A
|
A
|
A
|
R
|
R
|
R
|
R
|
R
|
G
|
G
|
G
|
| |
2
| |
A
|
A
|
A
|
R
|
R
|
R
|
R
|
R
|
G
|
G
|
G
|
| |
3
| |
A
|
A
|
A
|
R
|
R
|
R
|
R
|
R
|
G
|
G
|
G
|
| |
1
|
660
|
G
|
G
|
G
|
G
|
S
|
S
|
S
|
S
|
C
|
C
|
C
|
| |
2
| |
G
|
G
|
G
|
G
|
S
|
S
|
S
|
S
|
C
|
C
|
C
|
| |
3
| |
G
|
G
|
G
|
G
|
S
|
S
|
S
|
S
|
C
|
C
|
C
|
- Dilution series were created from mixtures of bacterial 16S amplicons obtained from the following Osedax samples: (A) T1048.115 clone E6; (B) T1048.115 clone D11; (C) T1071.13 clone D4; (D) T1071.13 clone C6; (E) worm T1071.35; (F) worm T1048.110. Each mixture was replicated three times. Mixtures detected by sequencing software are marked in boldface.
