Figure 4

Blast-based mapping of modification sites for all eukaryotic C/D snoRNAs in Rfam. A) An example slice is shown, where colors correspond to individual Rfam families/clans and positions traceable to the LECA are denoted by asterisks. B) For SSU rRNA, we identified 23 LECA snoRNAs performing LECA-conserved 2'-O-methylation (13 families). For LSU rRNA, 21 LECA snoRNAs perform LECA-conserved 2'-O-methylation (18 families). The overlap between both data sets contained two familes. Note that, of the original 42 LECA families, four were not considered in this analysis because they either did not belong to the C/D box class (2) or are known to function exclusively in rRNA cleavage (2). Complete results are provided in supplementary material (Additional file 1: Table S3).