Table 5 All primers used for PCR amplification and sequencing in this study
Marker | Primer name | Forward primer sequence | Annealing temperature (°C) | Reference |
|---|---|---|---|---|
ITS | ITS1F | 5’-CTTGGTCATTTAGAGGAAGTAA-3’ | 55-60 | [113] |
|  | ITS_Mel_F | 5’- TGCTTTGGCGGRYCYYRRG-3’ | 55-60 | This study |
|  | ITS4 | 5’-TCC CCGCTTATTGATATGC-3’ | 55-60 | [114] |
|  | ITS4A | 5’- CGCCGTTACTGGGGCAATCCCTG-3’ | 55-60 | [115] |
|  | ITS4 | 5’-TCCTCCGCTTATTGATATGC-3’ | 55-60 | [114] |
LSU | Al2R | 5’-GCGAGTGAAGCGGCAACAGCTC3’ | 55-60 | [116] |
|  | LR3 | 5’-CCGTGTTTCAAGACGGG-3’ | 55-60 | Vilgalys unpublished |
mtSSU | mrSSU1 | 5’-AGCAGTGAGGAATATTGGTC-3’ | 55-60 | [117] |
|  | mrSSU3R | 5’-ATGTGGCACGTCTATAGCCC-3’ | 55-60 | [117] |
MCM7 | Mcm7-709for | 5’-ACIMGIGTITCVGAYGTHAARCC-3’ | 56 | [112] |
|  | Mcm7-1348rev | 5’-GAYTTDGCIACICCIGGRTCWCCCAT-3’ | 56 | [112] |
|  | X_Mcm7_F | 5’-CGTACACYTGTGATCGATGTG-3’ | 56 | [118] |
|  | X_Mcm7_R | 5’- GTC TCC ACG TAT TCG CAT TCC-3’ | 56 | [118] |
RPB1 | gRPB1-A for | 5’-GAKTGTCCKGGWCATTTTGG-3’ | 54-56 | [119] |
|  | fRPB1-C rev | 5’-CCNGCDATNTCRTTRTCCATRTA-3’ | 54-56 | [119] |
|  | RPB1_MH_F | 5’-ACGTCGCCGAGACCCHAARA-3’ | 54-56 | This study |
RPB2 | RPB2-6F | 5’-TGGGGKWTGGTYTGYCCTGC-3’ | 50-56 | [120] |
|  | fRRPB2-7cR | 5’-CCCATRGCTTGYTTRCCCAT-3’ | 50-56 | [120] |
|  | RPB2_MH_F | 5’-ACAGTCGGTACWCCCAGYGAGCC-3’ | 50-56 | This study |
|  | RPB2_MH_R | 5’-TGCCCATAGCCGATTGGTAYGTATT-3’ | 50-56 | This study |