ML phylogenetic tree of Physarales isolates based on the LSU data set. The analysis was based on the alignment of 765 nucleotide positions from 57 isolates representing the Didymiaceae and Physaraceae families. The evolution models are TrNef + I (Didymiaceae) and GTR + I + G (Physaraceae). Tree reconstruction using NJ, MP, ML, or BAY resulted in essential similar tree topologies. Bootstrap values (> 50%) of 2000 replications are shown above the branches (NJ/MP/ML). Node of high statistical support (bootstrap values of 100%) is indicated by red circle. BAY analysis consists of 1,000,000 generations with trees sampled every 100 generations. BAY posterior probabilities are shown below branches, and branches with posterior probabilities of 1.0 are presented as thick black lines. A. ML-tree of Didymiaceae isolates. P. polycephalum was used as an out-group. B. ML-tree of Physaraceae isolates. D. iridis was used as an out-group.