Tissue-specific differential induction of duplicated fatty acid-binding protein genes by the peroxisome proliferator, clofibrate, in zebrafish (Danio rerio)

Table 2 Composition of diets (% by weight)

Ingredients	0% clofibrate diet	0.25% clofibrate diet	0.50% clofibrate diet	0.75% clofibrate diet	1.00% clofibrate diet
Vitamin free casein^a	33	33	33	33	33
Wheat gluten^b	10	10	10	10	10
Gelatin^a	4	4	4	4	4
Corn oil^c	4	4	4	4	4
Fish oil^d	4	4	4	4	4
Corn starch^e	33	33	33	33	33
Celufil¹	8.00	7.75	7.50	7.25	7.00
Vitamin mix^f	1.30	1.30	1.30	1.30	1.30
Mineral mix^g	1	1	1	1	1
Betaine^h	1.50	1.50	1.50	1.50	1.50
DL-Methionine^a	0.20	0.20	0.20	0.20	0.20
Clofibrateⁱ	0	0.25	0.50	0.75	1.00
Total	100	100	100	100	100

^a US Biochemical. (Cleveland, OH, USA).
^b Dover Mills Ltd. (Halifax, NS, Canada).
^c Sobeys Inc. (Halifax, NS, Canada).
^d Corey Feeds Ltd. (Fredericton, NB, Canada).
^e National Starch and Chemical Co. (Bridgewater, NJ, USA).
^f Vitamin added to supply the following (per kg diet): vitamin A, 8000 IU; vitamin D3, 4000 IU; vitamin E, 300 IU; menadione sodium bisulfite, 40 mg; Thiamine HCl, 50 mg; riboflavin, 70 mg; d-Ca pantothenate, 200 mg; biotin, 1.5 mg; folic acid, 20 mg; vitamin B12, 0.15 mg; niacin, 300 mg; pyridoxine HCl, 20 mg; ascorbic acid, 300 mg; inositol, 400 mg; choline chloride, 2000 mg; butylated hydroxy toluene, 15 mg; butylated hydroxy anisole, 15 mg.
^g Mineral added to supply the following (per kg diet): manganous sulphate (32.5% Mn), 40 mg; ferrous sulphate (20.1% Fe), 30 mg; copper sulphate (25.4% Cu), 5 mg; zinc sulphate (22.7% Zn), 75 mg; sodium selenite (45.6% Se), 1 mg; cobalt chloride (24.8% Co), 2.5 mg; sodiumfluoride (42.5% F), 4 mg.
^h Betaine anhydrous (96% feed grade). (Finnfeeds, Finland). ⁱ Sigma-Aldrich Inc. (St. Louis, MO, USA).

ISSN: 2730-7182