Phylogenetic trees of (A) PTHRs and (B) PTH family peptides. Consensus trees were constructed using Maximum Likelihood (ML) and 500 bootstrap replicates. Bootstrap values higher than 50% confidence are mapped. In A) rooted tree of the 31 taxa deduced mature protein sequences of the genes represented in Table 2 with human SCTR (HsaSCT) as outgroup. Chicken PTH1R and PTH3R are in bold and the Platypus PTH1R, the putative shark members and lamprey PTH1R were not utilized due to short predicted sequence. In B) unrooted tree of the deduced mature precursor sequence of PTH-family members from 35 taxa. Accession numbers: Human (PTH, AAH96144; PTHrP, AAA60216, TIP39 NP_848544); Mouse (PTH, NP_065648; PTHrP, CAC39218; TIP39 NP_444486); Chicken (PTH NM_205452, PTHrP NC_006092, PTH-L, CAW30790), Lizard (PTH ENSACAP00000018344; PTHrP ENSACAP00000018097); Xenopus (PTH FM955441, PTHrP NM_205338, TIP39 ENSXETP00000057310); Zebrafish (PTH1, NP_998115; PTH2, NP_998114; PTHrPA, AAY87956; PTHrPB, AAY87957; PTH-L, CU856139, TIP39 NP_991140); Takifugu (PTH1, CAG26460; PTH2, CAG26461; PTHrPA, CAB94712; PTHrPB, CAG26459); Seabream PTHrP AF197904_1; Elephant shark (Pth1 ADJ218, Pth2 ADJ21811, PthrP ADJ21812). Sequences from Opossum PTH_L (chromosome 2), Lizard TIP39 (GL343355.1), Elephant shark TIP39 (AAVX01305587.1), Lamprey Pth/PthrP (GL476611) and TIP39 (GL477315) were deduced from the genome sequence.