Spore development over time. A and B, Intercalary swellings along the double-walled hypha give rise to a spore primordium (arrow in A). C and D, 15 day-old juvenile spore with subtending hypha and a septum in the mother hypha (arrow in C). E and F, Intercalary 30 day-old spores with subtending hypha and bi-layered spore wall. G, Crushed 60 day-old spore with subtending hypha and separating spore wall layers. The apical portion of the mother hypha has emptied, septated and detached from the spore (arrow in E). H, 60 day-old spore observed with a confocal microscope. I and J, 90 day-old mature spore still attached to subtending hyphae. The images in panels A, C, E, G and I were taken using a DIC optical microscope. The merged images in panels B, D, F, H and J were taken using a confocal microscope where nuclei were stained with SytoGreen fluorescent dye. Scale bars represent 10 μm. K, Spore diameter plotted against spore age using a quadratic model (y = -0,0107X2 + 2,1243X + 5,9132).