Similarities between malin and the TRIM family of proteins. A) Schematic depicting the domains present in malin, TRIM2, TRIM32, TRIM56, and TRIM71. All domains and domain placement are to scale, and based on analyses using PROSITE and PFAM. B) The RING domain of malin, TRIM2, TRIM32, and TRIM56 were aligned and analysed for percent similarity and identity. C) CLUSTALX alignment of the RING domains of malin, TRIM2, TRIM32, and TRIM56. D) Phylogenetic relationships between malin and TRIM proteins in mammals. Sequences of rat (R. norvegicus), mouse (M. musculus), human (H. sapiens) and chimp (P. troglodytes) orthologs for malin and related TRIM proteins, obtained after BLASTP searches, were aligned using MAFFT (additional file 5, Figure S4) and used for the construction of a maximum-likelihood phylogenetic tree as described in Material and Methods.