Coalescent-based genealogy of the internal transcribed spacers (ITS1 and ITS2), showing the distribution mutations for New World (Pacific, WA = Washington State; Mountain USA, WY = Wyoming, CO = Colorado) and Old World (EU = Europe) populations of C. aureofulvus performed using Genetree. The inferred genealogy is based on five million simulations of the coalescent. Simulations were carried out assuming a constant population size. Maximum likelihood estimates of the tree with the highest root probability (likelihood = 1.75 × 10-8) and standard deviation (SD = 1.61 × 10-6) showing the distribution of mutations. The direction of divergence is from the top of the genealogy (oldest = past) to bottom (youngest = present); coalescence is from the bottom (present) to the top (past). The numbers below each branch indicate the corresponding haplotype and its frequency in each macro-population and sampling area. The time scale is in coalescent units of effective population size.