Figure 5From: The two tryptophans of β2-microglobulin have distinct roles in function and folding and might represent two independent responses to evolutionary pressureEquilibrium GdnHCl denaturation curves of wild-type and Trp95Leu β2-m. GdnHCl denaturation of protein was monitored at pH 7.4 and 20°C by far UV circular dichroism (215 nm). Ellipticities were converted to the apparent unfolded fraction as described in the Methods section.Back to article page