Figure 4

LBD alignment. The alignment of the LBDs of the MRs and GRs used in this study demonstrates the high sequence identity between genes. Sequence locations of the secondary structure helices are given below the alignment. To identify amino acids that may play a role in mineralocorticoid sensitivity of the duplicate teleost GRs, we aligned the LBDs of GRs that are mineralocorticoid insensitive (green) and GRs that show mineralocorticoid sensitivity (blue). We identified nine positions (highlighted within the alignment) with amino acid residues conserved within but not between aldosterone-sensitive and insensitive groups. These residues included positions 15, 38, 49, 129, 161, 195, 203, 204 and 235. (Position 1 of the LBDs corresponds to positions 538 and 532 of the full-length mouse and human GRs, respectively.)