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Table 1 Genetic diversity estimated using DNASP for each locus: Hd, haplotype diversity; S, number of segregating sites and π, nucleotide diversity.

From: Diversification across an altitudinal gradient in the Tiny Greenbul (Phyllastrephus debilis) from the Eastern Arc Mountains of Africa

Locus Nbp/Nvar Population Nalleles θ (from S) Nhaplotype Hd S π Fu's Fs/R 2
ND2 1041/170 debilis sensu lato 110 0.03106 43 0.948 170 0.05776 11.200/0.1757
   albigula (Nguru + Usambara Mts) 59 0.00539 13 0.844 25 0.00586 1.303/0.1175
   Nguru Mts 38 0.00161 6 0.751 7 0.00215 1.120/0.0839
   Usambara Mts 21 0.00375 7 0.562 14 0.00239 -0.458/0.1553
   Lowland (rabai + debilis sensu stricto) 51 0.02034 30 0.965 95 0.02068 -1.521//0.1103
   rabai 41 0.01216 22 0.948 54 0.00833 -3.976**/0.0740
   debilis sensu stricto 10 0.00579 8 0.956 17 0.00681 -1.104/0.1827
FGB 559/17 debilis sensu lato 184 0.00525 17 0.575 17 0.00432 -3.383*/0.0706
   albigula (Nguru + Usambara Mts) 118 0.00067 3 0.05 2 0.00009 -3.527*/0.0466*
   Nguru Mts 76 0.00036 2 0.052 1 0.00009 -1.248/0.0260**
   Usambara Mts 42 0.00042 2 0.048 1 0.00009 -1.149/0.1525
   Lowland (rabai + debilis sensu stricto) 66 0.00564 15 0.777 15 0.00460 -4.127**/0.0852
   rabai 58 0.00425 10 0.713 11 0.00401 -1.198/0.1018
   debilis sensu stricto 8 0.00483 6 0.929 7 0.00530 -1.637/0.1866
GAPDH 328/15 debilis sensu lato 208 0.00776 20 0.827 15 0.00622 -7.122**/0.0676
   albigula (Nguru + Usambara Mts) 110 0.00464 11 0.824 8 0.00619 -1.261/0.1266
   Nguru Mts 68 0.00511 10 0.824 8 0.00683 -0.934/0.1397
   Usambara Mts 42 0.00355 6 0.661 5 0.00329 -1.016/0.1074
   Lowland (rabai + debilis sensu stricto) 98 0.00771 16 0.556 13 0.00351 -10.918**/0.0439
   rabai 70 0.00762 15 0.634 12 0.00443 -8.485**/0.0596
   debilis sensu stricto 28 0.00157 3 0.320 2 0.00103 -0.731/0.0840
BRM 364/12 debilis sensu lato 168 0.00530 14 0.849 11 0.00768 -0.914/0.1257
   albigula (Nguru + Usambara Mts) 96 0.00214 4 0.639 4 0.00226 0.852/0.1100
   Nguru Mts 62 0.00264 4 0.633 4 0.00264 0.947/0.1284
   Usambara Mts 34 0.00067 2 0.428 1 0.00118 1.407/0.2139
   Lowland (rabai + debilis sensu stricto) 72 0.00397 10 0.815 7 0.00511 -1.598/0.1328
   rabai 58 0.00415 9 0.759 7 0.00508 -1.221/0.1321
   debilis sensu stricto 14 0.00259 4 0.692 3 0.00353 0.135/0.2143
  1. In the absence of selection, significant negative values of Fu's Fs and low values of R 2 are indicative of population expansion (*p < 0.05, **p < 0.01). For the three nuclear loci, only alleles with phase probabilities greater than 0.75 were included in the analyses. The subspecies rabai includes all individuals from lowland Tanzania (including the three specimens from SE Tanzania, whereas subspecies debilis only includes individuals from Mozambique and Zimbabwe, see text for details).