BMC Ecology and Evolution

Table 1 Intraspecific polymorphism and neutrality tests for the three Anopheles short PGRP genes.

From: Molecular evolution of the three short PGRPs of the malaria vectors Anopheles gambiae and Anopheles arabiensisin East Africa

		5' upstream			Exon 1														3' downstream
PGRP	Population	sequence			UTR			Translated region				Intron			Exon 2				sequence			Total				Neutrality
		L	S	π	L	S	π	L	S		π	L	S	π	L	S		π	L	S	π	L	S	π	πa/πs	Tajima's	Fu & Li
									s	n						s	n									D	D'	F
	Ag_Tz		5	0.002		-	-		0	1	0.000		1	0.003		1	0	0.001		0	0.000		8	0.001	0.338	-1.03*	-2.32*	-2.51*
	Ag_Mz		1	0.000		-	-		0	0	0.000		0	0.000		0	0	0.000		0	0.000		1	0.000	-	-1.11 n/s	-1.81 n/s	-1.86 n/s
S1	Aa Tz	534	1	0.000	-	-	-	255	0	0	0.000	74	0	0.000	297	0	0	0.000	32	0	0.000	1192	1	0.000	-	-1.16 n/s	-1.45 n/s	-1.57 n/s
	Aa Mz		31	0.013		-	-		3	0	0.003		1	0.004		4	1	0.004		3	0.023		43	0.008	-	-1.04 n/s	1.19 n/s	0.65 n/s
	Ag_Tz		8	0.039		19	0.021		14	3	0.014		-	-		-	-	-		9	0.027		54	0.020	0.073	2.50*	1.19 n/s	1.82***
	Ag_Mz		10	0.041		27	0.022		18	3	0.014		-	-		-	-	-		17	0.038		75	0.022	0.049	0.34 n/s	0.62 n/s	0.62 n/s
S2	Aa Tz	107	1	0.002	409	5	0.005	567	11	1	0.007	-	-	-	-	-	-	-	172	14	0.024	1255	32	0.009	0.030	-0.34 n/s	-0.39 n/s	-0.43 n/s
	Aa Mz		5	0.023		7	0.007		9	2	0.007		-	-		-	-	-		6	0.015		29	0.010	0.061	0.86 n/s	0.89 n/s	0.99/s
	Ag_Tz		1	0.001		-	-		10	1	0.003		-	-		-	-	-		0	0.000		12	0.002	0.015	-1.53 n/s	-1.78 n/s	-1.99 n/s
	Ag_Mz		10	0.043		-	-		28	10	0.025		-	-		-	-	-		6	0.019		54	0.027	0.141	1.08 n/s	1.21 n/s	1.38 n/s
S3	Aa Tz	84	8	0.038	-	-	-	567	16	1	0.007	-	-	-	-	-	-	-	61	6	0.028	712	31	0.012	0.038	-0.08 n/s	0.31 n/s	0.22 n/s
	Aa Mz		10	0.014		-	-		23	9	0.010		-	-		-	-	-		5	0.019		47	0.011	0.042	-1.66 n/s	-1.97 n/s	-2.19 n/s

*, **, ***represent P < 0.05, P < 0.01, and P < 0.001, ^n/s not significant. Ag_Tz, An. gambiae from Tanzania; Ag_Mz, An. gambiae from Mozambique; Aa_Tz, An. arabiensis from Tanzania; Aa_Mz, An. arabiensis from Mozambique; L, length; S, segregating site n - total number of nonsynonymous changes; s - total number of synonymous changes; π, nucleotide diversity.

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