Figure 3

Phylogenic tree of tCWCH2. (A) Tree indicating similarities among the tCWCH2 gene classes. Statistical analysis was performed using three ZFs (tCWCH2 and a ZF in the C-terminal flanking region). (B) Sub-tree of Zic/Gli/Glis class gene. The alignment for this analysis is shown in Additional file 11. BI, NL, and NJ analyses were carried out to construct the molecular phylogenetic trees (Additional files 12, 13 and 14). The tree pattern is based on the BI tree. Scores for each branch indicate the statistical support values obtained in each phylogenetic tree construction method {BI (postprobability)/ML (bootstrap value)/NJ (bootstrap value)}. The absence of scores (-) indicates the branches that were not supported by the corresponding methods.