Table 3 The primers used to amplify the four overlapping long range amplicons.
From: Evolutionary relationships and divergence times among the native rats of Australia
A. | Â | Â |
|---|---|---|
Primer name | Sequence (5' to 3') | |
Av175312SF | AAACTGGGATTAGATACCCCACTAT | |
R6036R | ACTTCTGGGTGTCCAAAGAATCA | |
BatL5310 | CCTACTCRGCCATTTTACCTATG | |
LR2RB | CTGATTGGAAGTCAGTTGTATTTTT | |
L10647F | TTTGAAGCAGCAGCCTGATAYTG | |
RCb9H | TACACCTAGGAGGTCTTTAATTG | |
RGlu2L | CAGCATTTAACTGTGACTAATGAC | |
Long 16SR | TGATTATGCTACCTTTGCACGGTCAGGATACC | |
B. | Â | Â |
Region amplified | Primer pairs | Amplicon length |
12S - COI | Av175312sf & R6036R | 5.55 kb |
COI - COIII | BatL5310 & LR2RB | 4.11 kb |
COIII - Cyt b | L10647F & RCb9H | 5.53 kb |
Cyt b - 16S | RGlu2L & Long 16SR | 4.26 kb |