Figure 3

The maximum-likelihood phylogenetic tree of LpxH, LpxH2, and related proteins. Circles at internal nodes indicate bootstrap-supporting values as follows: black circles ≥ 95%, gray circles ≥ 85%, and white circles ≥ 70%. Proteobacteria are shown with green letters and lines. Group-II Gammaproteobacteria are indicated by *. See Figure 2 legend for abbreviations used for bacterial classification. The red arrowhead shows where the LpxH/LpxH2 duplication happened. The inset shows the maximum likelihood phylogeny reconstructed from all of the similar proteins identified in this study. It shows that each protein family (indicated with triangles with InterPro accession numbers) forms a distinct cluster.