Figure 3

A trans-NAT expressed antisense of a pseudogene. (A) Genomic features in a region of human chromosome 3 (negative strand, coordinates decrease from left to right). EST AI803540 is expressed in this region from positions 75,547,155 to 75,547,600 and represents a trans-NAT expressed antisense of a pseudogene. Another five ESTs have an identical or very close 3'-end position and support the same antisense transcript. "Pooled" indicates that the EST was obtained from pooled human melanocyte, fetal heart, and pregnant uterus. (B) This genomic region is highly identical to the parental gene region in Chromosome 16, from positions 5,068,153 to 5,068,597, as indicated by sequence alignment (partially represented in the figure). The PAS of the trans-NAT ("AATAAA", in yellow) is not conserved in the parental gene. (C) The region of similarity is located in an intron of gene ALG1 and it is antisense of the direction of transcription. No evidence of antisense transcription was found in this region of the parental gene and some mutations happened in the positions aligning to the PAS of the trans-NAT.