Figure 7

Ci-Syn expression at the larval stage. Whole mounts in side view, except for E', J, O in dorsal view, anterior is left. Scale bars = 50 μm in whole mounts; 20 μm in cross sections. A-N': Newly hatched larva. A: Side view, the signal extends from the sv until two/third of nerve cord (nc). A': Enlargement of the tail. Ci-Syn is expressed in three bipolar neurons (arrowheads). Small spots are also visible along the nc (arrows). B, B': Cross sections through levels in A' showing the dorsolateral fibers (arrows) and a bipolar neuron (arrowhead). Moreover, a small positive dot is visible. C: Lateral view. Expression is found in sv, ne, vg, neurons of adhesive papillae and some RTEN. C'-D: Cross sections through levels in C showing the expression in papillar neurons. D', E: Cross sections through levels in C. Ci-Syn expression occurs in some RTEN (arrowheads). E': Dorsal view of the specimen in A showing expression in several regions of the sv. Expression is also detected in the ne and the vg. F-I': Cross sections through levels in E'. J: Enlargement of specimen in C in side view. J', K: Cross sections through levels in J. J': Transcripts are in the ventral neck region. K: A signal is found in ventral motor neurons and in dorsal cp. K': Enlarged dorsal view of the tail. Red arrowheads indicate anterior caudal inhibitory neurons (ACINs); arrows indicate motor neuron endplates. L-N': Cross sections through levels in K'. Ci-Syn is expressed in ACINs and in ventrolateral elements (arrowheads). O-O': Late larva. O: Dorsal view, the arrow indicates one RTEN; arrowheads indicate bipolar neurons. O': Enlargement of the trunk showing positive RTEN (arrows). cp, contrapelo cells; coronet cells (green arrow), ec, eminens cell; ne, neck; ph, pharynx; pigment cell of ocellus (red arrowhead in E'and G-I); sv, sensory vesicle; vg, visceral ganglion; white asterisk, pigment cell of otolith.