Genomic organization and gene expression of the multiple globins in Atlantic cod: conservation of globin-flanking genes in chordates infers the origin of the vertebrate globin clusters

Table 2 Primers for real-time qPCR, amplification efficiency (%) and amplicon size (bp).

Gene	Name	Sequence (5' to 3')	Efficiency	Size
α1	A1F	GACTTACTTCAGCCACTGGAAGAGCCT C	96	153
	A1R	TTGAAGGCGTGCAGCTCGCTCAGAG
α2	A2F	GTCCTATTTCTCTCACTGGAAGGACGCG	85	153
	A2R	ATGAACGCGTGCAGCTCGCTAAGGC
α3	A3F	CACATCATACCCTGGCACCAAGAC	95	172
	A3R	CTGGTAGGCGTGGTAGGTTTGAAGAG
α4	A4F	TTCTCCCACTGGAAAGACCTCGG	70	138
	A4R	ATGGAGCTCACTGAGCTCGAGAAG
β1 allele A	B1FA	TTATGGGAAACCCCAAGGTGGCCAA	91	131
	B1R	GTGCAGTTTCTCGGAGTGCAGCACGC
β1 allele B	B1FB	TTGTGGGAAACCCCAAGGTGGCTGC	98	131
	B1R	GTGCAGTTTCTCGGAGTGCAGCACGC
β2	B2F	CCTGTACAATGCAGAGACCATCATGGC	84	151
	B2R	GTGCAGCTTGTCAGAGTGGAGCAGAG
β3	B3F	ACAGATAGTGAGCGCGCCATCATTAA	86	176
	B34R	GCGGCGATCAGGGGGTTGCACAG
β4	B4F	ACAGATAGTGAGCGCGCCATCATTAC	95	176
	B34R	GCGGCGATCAGGGGGTTGCACAG
β5	B5F	GTGGACTCGGAGGTACTTTGGAAAC	89	168
	B5R	TGCAGCTGACTGAGCTCGCAATAG
Ubiquitin	UbiF	GGCCGCAAAGATGCAGAT	81	69
	UbiR	CTGGGCTCGACCTCAAGAGT

ISSN: 2730-7182