The evolution of vertebrate tetraspanins: gene loss, retention, and massive positive selection after whole genome duplications

Table 2 Statistics on branches under significant positive selection

Branch type	No. of branches	Proportion of sites under positive selection average(median)	Significant LRT^a (P < 0.05) (proportion)	Fisher's exact test^b	Significant LRT^a (P < 0.01) (proportion)	Fisher's exact test^b
* 13 tested trees, CD63, TSPAN8, TSPAN10 and TSPAN12 excluded
A. duplications	34	-	26(76%)	0.0425;A/B	19(56%)	0.0045;A/B
B. mammals^c (portion 1,2,3 as a whole)	31	-	15(48%)	0.7961;B/D	6(19%)	0.7552;B/D
C. mammals^c (portion 1,2,3 as independent branches)	81	-	26(32%)	1.4e-5;A/C	13(16%)	3.0e-5;A/C
D. teleosts	28	-	15(54%)	0.0667;A/D	7(25%)	0.0202;A/D
* 15 tested trees, TSPAN8 and TSPAN12 excluded
E. duplications	36	18.4%(19.0%)	28(78%)	0.0138;E/F	20(56%)	0.0025;E/F
F. mammals^c (portion 1,2,3 as a whole)	33	-	16(48%)	0.8025;F/H	6(18%)	0.7577;F/H
G. mammals^c (portion 1,2,3 as independent branches)	86	7.2%(4.1%)	27(31%)	3.0e-6;E/G	14(16%)	0.0001;E/G
H. teleosts	30	9.6%(8.3%)	16(53%)	0.0652;E/H	7(23%)	0.0118;E/H
* TSPAN8 and TSPAN12 (Though the branch-site model detects no positive selection, the site-specific model detects positive selection on TSPAN8)
I. duplications	0	-	-	-	-	-
J. speciation (as independent branches)	6	-	0(0%)	-	0(0%)	-

^aNumber of branches on which positive selection is detected using branch-site model at given p-value; Hochberg's method (1988) was used for multiple testing correction.
^bFisher's exact test was used for testing the difference between different types of branches
^cThe bony vertebrate-mammal branch was interrupted by amphibians and reptiles, hence produced portion 1,2,3, see Figure 5A for details.

ISSN: 2730-7182