From: Evolution of AANAT: expansion of the gene family in the cephalochordate amphioxus
Substrate | ||||||||
---|---|---|---|---|---|---|---|---|
Phenylethylamine | Tyramine | Serotonin | Tryptamine | |||||
bfAANAT | α | δ' | α | δ' | α | δ' | α | δ' |
V max | 17.8 ± 1.1 | 4.9 ± 3 | 2.06 ± 0.28 | 0.78 ± .06 | 1.60 ± 0.1 | 0.56 ± 0.06 | 4.7 ± 0.8 | 2.7 ± 0.25 |
K m | 3 ± 0.6 | 45 ± 36 | 8.2 ± 2.70 | 0.80 ± 0.28 | 0.01 ± 0.01 | <0.01 | 8.1 ± 3.6 | 2.5 ± 0.7 |
Substrate | ||||||||
Octopamine | Propylamine | Butylamine | Putrescine | |||||
bfAANAT | α | δ' | α | δ' | α | δ' | α | δ' |
V max | 2.1 ± 0.2 | 0.80 ± 0.05 | >10 | 0.73 ± 0.07 | 32.5 ± 4.1 | 0.7 ± 0.05 | 1.34 ± 0.32 | 5.11 ± 0.11 |
K m | 6.8 ± 1.9 | 0.05 ± 0.01 | >20 | 0.90 ± 0.37 | 47.5 ± 8.0 | 0.37 ± 0.23 | 5.2 ± 4.0 | 3.68 ± 0.22 |