9.0) optimal pH, more than two pH units higher than that of vertebrate AANATs. Conclusions The substrate selectivity profiles of bfAANATα and δ' are relatively broad, including alkylamines, arylalkylamines and diamines, in contrast to vertebrate forms, which selectively acetylate serotonin and other arylalkylamines. Based on these features, it appears that amphioxus AANATs could play several roles, including detoxification and biogenic amine inactivation. The presence of seven AANATs in amphioxus genome supports the view that arylalkylamine and polyamine acetylation is important to the biology of this organism and that these genes evolved in response to specific pressures related to requirements for amine acetylation."/>
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Figure 6 | BMC Evolutionary Biology

Figure 6

From: Evolution of AANAT: expansion of the gene family in the cephalochordate amphioxus

Figure 6

Comparison of acetylation activity of expressed vertebrate and amphioxus AANATs. Acetylation activity of GST-bfAANATα, GST-bfAANATδ' (both at pH 9.0) and GST-oAANAT (at pH 6.8) towards various substrates (10 mM) was determined. PUT: putrescine, PPA: propylamine, BTA: butylamine, PEA: phenylethylamine, TRP: tryptamine. Data are presented as the mean ± S.E.M (N = 3). For further details see Methods.

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