A juvenile bird with possible crown-group affinities from a dinosaur-rich Cretaceous ecosystem in North America

Background Living birds comprise the most speciose and anatomically diverse clade of flying vertebrates, but their poor early fossil record and the lack of resolution around the relationships of the major clades have greatly obscured extant avian origins. Results Here, I describe a Late Cretaceous bird from North America based on a fragmentary skeleton that includes cranial material and portions of the forelimb, hindlimb, and foot and is identified as a juvenile based on bone surface texture. Several features unite this specimen with crown Aves, but its juvenile status precludes the recognition of a distinct taxon. The North American provenance of the specimen supports a cosmopolitan distribution of early crown birds, clashes with the hypothesized southern hemisphere origins of living birds, and demonstrates that crown birds and their closest relatives coexisted with non-avian dinosaurs that independently converged on avian skeletal anatomy, such as the alvarezsaurids and dromaeosaurids. Conclusions By revealing the ecological and biogeographic context of Cretaceous birds within or near the crown clade, the Lance Formation specimen provides new insights into the contingent nature of crown avian survival through the Cretaceous-Paleogene mass extinction and the subsequent origins of living bird diversity. Supplementary Information The online version contains supplementary material available at 10.1186/s12862-024-02210-9.


Introduction
Birds form the largest radiation of flying vertebrates and include over ten thousand living species that display exceptional anatomical, behavioral, and ecological diversity.Although their dinosaur origins are now firmly established, the early evolution of the avian crown remains poorly known.This gap in the evolutionary history of living birds is nearly unparalleled among other major vertebrate crown clades [1][2][3][4][5] and is largely attributable to two factors.First, living birds have an exceptionally poor early fossil record.Few unambiguous crown birds are known from the Mesozoic [2,[6][7][8][9], and the fossil records of most major living avian lineages are limited to a handful of fossils or wholly nonexistent [10].Second, analyses of both massive morphological [11] and genomic datasets have failed to produce a consilient picture of the interrelationships of major living bird clades, presenting one of the greatest challenges in phylogenetics [12][13][14][15][16][17][18].
Here, I use computed tomography to describe a probable Mesozoic crown bird based on an associated skeleton of a juvenile individual from the latest Cretaceous Lance Formation of Wyoming.The provenance of this specimen provides new evidence against the classically hypothesized southern hemisphere origins of living birds.The prehistoric ecosystem inhabited by the crown bird included numerous representatives of extinct stembirds and bird-like dinosaurs ( [19]; this paper), providing evidence for the direct coexistence of non-avian theropods and the earliest representatives of the extant avian radiation.

Description
Yale Peabody Museum Vertebrate Paleontology Collections (YPM VP) 59473, a partial skeleton consisting of the complete left quadrate, portions of the skull roof a partially articulated, though very poorly preserved, cervical series, a fragment of the synsacrum, the left humerus, the articulated left radius and ulna, partial left tibiotarsus, and partial pes.The specimen (Figs. 1, 2, 3, 4 and 5) is preserved in course-grained sediment that also included a small non-avian theropod tooth (identifiable based on the presence of serrations, and plausibly assignable to Tyrannosauroidea or Dromaeosauridae; Supplementary Text III).The bones are all closely associated or articulated (radius and ulna) in three small (< 5 cm) long blocks of conglomerate.Because no bones overlap and all show the same ontogenetic indicators (striated bone texture), they most likely come from a single individual.All bones except for the humerus, tibiotarsus, and a large partial pedal phalanx are embedded in matrix.Matrix portions also still cling to spots on these prepped-out bones.The quadrate was embedded in what appears to be an iron or bone-flake rich region of sediment in the block, and so its external surfaces appear less well preserved on the CT scan render than other bones.

Locality and horizon
Niobrara County, Wyoming, United States, North America.The fossil was collected from the Lance Formation by the J.B. Hatcher expedition, 1890-1904.The Lance Formation is a Maastrichtian-age unit that crops out across

Referral
Crown avian and galloanserine affinities for YPM VP 59473 are supported by: clear separation of the otic and squamosal capitula on the quadrate (Fig. 2d); presence of a subcapitular tuberculum below the squamosal capitulum on the quadrate (Fig. 2a); expansion of the ventral condyles and pterygoid condyle on the quadrate (Fig. 2a-c, e); humeral head dorsally offset from the rest of the proximal margin of the humerus (Fig. 3a, b); tricipital fossa is deeply excavated (Fig. 3a, b); dorsal tubercle of the humerus is large and offset from the rest of the proximal margin (Fig. 3a, b).

Ontogenetic assessment
Following the comprehensive review of osteological indicators of ontogenetic status presented in [20], I conducted a survey of YPM VP 59473 which revealed its juvenile status (Fig. 5).Features unambiguously indicating ontogenetic immaturity in birds present in this specimen include the presence of unfinished, heavily striated bone surfaces [20][21][22].Histological sections were not made due to the fragmentary nature of the specimen.Among the characters used to assign YPM VP 59473 to crown Aves and to Galloanserae, several deserve comment because they appear to change during ontogeny in stem and crown birds: the shape of the humeral head, development of the dorsal tubercle, and the depth of the tricipital fossa.In both juvenile and adult stem birds (for example, †Archaeorhynchus and †Ichthyornis), these features are weakly developed [23][24][25].In crown birds, the dorsal tubercle and the humeral head become progressively more prominent with ontogeny (for example, Phalacrocorax capillatus; Fig. 3A in [22].Thus, even accounting for the juvenile status of the specimen, the morphology of YPM VP 59643 is inconsistent with the hypothesis that it is a juvenile stem bird.

Description
YPM VP 59473 is from a small crown bird; size estimation using the regression equation for humerus length in [26] and an estimated length of 70.0 mm for the nearly complete humerus of YPM VP 59473 gives a value of 413.4 g, which is close to the mass of the †Asteriornis maastrichtensis holotype individual estimated using the same dataset [2].
Quadrate The left quadrate (Fig. 2a-e) is completely preserved, though slightly abraded along the lateral surface of its main body.The quadrate is similar to those of the early galloanserines †Asteriornis maastrichtensis (Fig. 2f-j) [2], †Presbyornis pervetus [27,28], and †Gallinuloides wyomingensis [29], but differs considerably from the quadrates of early anseriforms like †Conflicto antarcticus [2,30] or crown anseriforms and galliforms [2,28] such as Branta canadensis, Anas platyrhynchos, and Leipoa ocellata.A key apomorphy of Neognathae, the distinct separation of the otic and squamosal capitula at the dorsal end of the quadrate [2], is present in the quadrate of YPM VP 59473 (Fig. 2d).In stem-group ornithurans like †Ichthyornis dispar and †Apsaravis ukhaana, the capitula are not delimited by a deep intercapitular sulcus ([83]; [31]), and in Paleognathae, these form a single proximal process rather than two distinct apices ( [32]).This lack of separation is present in specimens of †Ichthyornis dispar that are of different sizes [31].Unlike the Lance Formation bird quadrate UCMP 53969 ( [28]), which possess an otic capitulum that is larger and more circular than the squamosal capitulum, the otic and squamosal capitula in the quadrate of YPM VP 59473 are of approximately equal size and shape (Fig. 2c, d).Similarly shaped capitula of equal size are also found in †A.maastrichtensis (Fig. 2h, i).
The main body of the quadrate in YPM VP 59473 is dorsoventrally straight and pillar-like, with slightly concave anterior and posterior margins.Unlike †Asteriornis maastrichtensis (Fig. 2g, h), †Presbyornis pervetus [28], †Conflicto antarcticus [2,30], the Lance 'galloanserine' quadrate described by [9], gastornithiforms [31][32][33], and crown galliforms [2,27,28], but similar to most crown anseriforms [2,28], the quadrate of YPM VP 59473 lacks any identifiable large foramina on the surface of its main body surrounding the orbital process (Fig. 2b,  c).A single foramen, the basiorbital foramen, is present in ornithurans close to the crown [34], suggesting that the absence of these in YPM VP 59473 is an autapomorphy.However, the phylogenetic polarity of quadrate main body foramina is unclear.Further, the juvenile status of YPM VP 59473 may imply that main body foramina were present but very small due to ontogeny, as may be the case for one of the foramina in †Asteriornis maastrichtensis [2].Further, damage to the surface of the quadrate in YPM VP 59473 might obscure the presence of foramina.The orbital process is developed into a triangular flange that expands from slightly over half of the dorsoventral axis of the quadrate main body in YPM VP 59473 (Fig. 2a-e), as in Ornithurae [2,28,34,35].
Based on the preserved bone surface, the orbital process is slightly shorter than in †Ichthyornis dispar [34] and †Conflicto antarcticus, but similar to the process in †Asteriornis maastrichtensis (Fig. 2f, g), Galliformes, and Anseranas semipalmata [2].However, the apex of the orbital process tends to be broken off in fossil quadrates, so the extent of this feature in YPM VP 59473 should be treated as uncertain.
The ventral end of the quadrate is developed into three major features: the lateral and medial condyles and what appear to be the base of the pterygoid condyle.The quadratojugal cotyla is positioned just dorsal to the lateral condyle, and consists of a deep, circular socket with a complete rim (Fig. 2a).The rim of the quadratojugal cotyla is socketed in crown galliforms and some crown anseriforms, but invariably lacks a notch in pan-anseriforms like †Presbyornis pervetus [2,27,28] and †Conflicto antarcticus [2,30], as well as the pan-galloanserine †Asteriornis maastrichtensis [2] and the Lance 'galloanserine' quadrate UCMP 53969 [9].
The bicipital crest of the humerus of YPM VP 59473 is large (Fig. 3a-d).This crest is marked on its dorsal surface by the transverse ligament sulcus, which is deep, clearly marked, and linear as in neornithines but unlike the condition in †Ichthyornis dispar [23,24], †Janavis finalidens [40], or †Apsaravis ukhaana [35], where the sulcus is shallow and rounded.The bicipital crest sits opposite to the deltopectoral crest, which is incompletely preserved.The deltopectoral crest is less distally extensive (measures less than 25% of the long axis of the humerus) than those of †I.dispar [23,24], †J.finalidens [40], †A.ukhaana [35], or †Tingmiatornis arctica [39], but compares favorably with †Vegavis iaii [6][7][8], †Antarcticavis capelambensis [37], and early-diverging crown neornithines [10,36,41].The caudal margin is also well developed in YPM VP 59473 relative to ichthyornithines and other stem birds close to the crown.†T.arcticus also possesses a strongly developed caudal margin of the humerus, despite sharing numerous features with ichthyornithines and not neornithines.The polarity of this character state remains unresolved even though neornithines appear to invariably show prominent caudal humerus margins.The distal end of the humerus of YPM VP 59473 is poorly preserved but does appear to show equally developed dorsal and ventral distal humeral condyles based on the shape and size of their medial portions (Fig. 3a-d).
Radius The radius (Fig. 3e-h) was preserved in articulation with the ulna on the surface of the larger of the two blocks included in the holotype of YPM VP 59473 (Fig. 1).The radius is very long and slender, with a straightened shaft (Fig. 3e-h).The surface of the radial shaft is heavily striated and lacks a finished surface, a feature indicative of the juvenile ontogenetic status of the holotype specimen of YPM VP 59473; this feature is also clearly present on the humerus, ulna, and distal tibiotarsus, although the radius is the best example of this texture on a bone still laying in situ.However, the medial surface of the radius, which is still encased in matrix, shows a conspicuous intermuscular line running down the shaft surface.This intermuscular line is found in living Aves and †Ichthyornis dispar [23,24].
The anterior surface of the distal end of the radius is marked by a large, round, and shallow sulcus identified as the tendinal groove.This feature lacks the prominent associated ridge found in extinct ornithurans like †Ichthyornis dispar [23] and is generally comparable to the weak tendinal groove found in neornithines (e.g., †Conflicto antarcticus, [30]).Posteriorly, there is a shallow ligamental depression and a weakly developed carpal facet.
Ulna The ulna (Fig. 3j-n) was preserved in articulation with the mostly complete radius on the surface of the larger of the two blocks included in the holotype of YPM VP 59473 (Fig. 1).The partial ulna, which includes at least one third of the shaft and the complete distal end, is uncrushed.The shaft is straight along the long axis of the ulna, curving only at its distal end to meet the radius at the wrist.The distal end of the ulna is approximately twice as wide mediolaterally as anteroposteriorly and has three major processes: the dorsal and ventral distal ulnar condyles and the carpal tubercle.Unlike †Ichthyornis dispar and stem birds within or close to Ornithurae [23,24,35], but similar to many neornithines (pers.obs., see specimens noted above), the carpal tubercle of the ulna is poorly developed and only differentiated from the rest of the bone by a shallow groove.The ovoid dorsal and ventral condyles are poorly developed and separated by a shallow intercondylar groove.Laterally, the surface of the dorsal condyle is marked by a shallow tendinal groove that runs proximodistally along the surface of the distal ulna.
Tarsometatarsus The shaft of the tarsometatarsus is preserved on the surface of the smaller block included in the holotype of YPM VP 59473 (Figs. 1, 4 and 5) and is identifiable as such based on its flattened, elliptical shaft, shallow extensor groove, and discrete lateral and medial margins (Fig. 4b).Little can be said about the morphology of this bone except that the distal end of the metatarsus appears to be more elongated than in †Ichthyornis dispar [23,24], hesperornithines (e.g., [43]), or †Apsaravis ukhaana [35], but similar to the Maastrichtian galloanserine †Asteriornis maastrichtensis [2], and the possibly anseriform presbyornithids [41,44,45].The possible Maastrichtian Antarctic neornithines †Vegavis iaii and †Antarcticavis capelambensis also all possess similarly elongated, fused tarsometatarsi such that the shaft of the bone shows little indication of each of the co-ossified bones [6][7][8]37].
Pes Six pedal phalanges and three possible pedal elements are preserved in the holotype of YPM VP 59473 (Fig. 1) with varying degrees of completeness (Fig. 6).
Two small, poorly preserved phalanges (Fig. 6a, b) are columnar and hollow internally, with slightly asymmetrical distal condyles separated by a deep intercondylar sulcus (Fig. 6a, b).However, the identification of these bones as phalanges is tentative, and they may be warped, fragmented portions of other long bones.One very elongated, slightly mediolaterally asymmetrical proximal phalanx (Fig. 6c) and the complete distal ungual (Fig. 6e) appear to represent either digits II or IV given their asymmetry.The ungual possesses a concave, elliptical proximal articular surface and a large, proximally positioned flexor tubercle on its ventral surface.The ungual body is weakly recurved.On the lateral and medial surfaces of the main body of the ungual, deep neurovascular grooves curve along the main axis.
Pedal digit III as reconstructed is represented by the distal third of the first phalanx, the proximal two-thirds of the second phalanx, and the complete third phalanx.These phalanges are bilaterally symmetrical and mediolaterally widened relative to the other bones of the pes.An asymmetrical distal end of the proximal phalanx (Fig. 6d) and a complete, poorly recurved, and slightly mediolaterally asymmetrical pedal ungual (Fig. 6f ) are also likely from digits II or IV.

Discussion
YPM VP 59473 sheds light on the biogeography of early crown birds and their closest relatives.Although stem birds are plentiful in the Late Cretaceous of North and South America [4,5,43,47,48], the record of Mesozoic crown birds from North and South America is restricted to a number of isolated bones of unclear phylogenetic position and provenance [4,9].
The discovery of YPM VP 59473 provides additional evidence that bird species very near to and within the crown clade acquired a cosmopolitan distribution by the Late Cretaceous, which is logical given the inferred capability for flight in YPM VP 59473 and other derived ornithurans like †Ichthyornis dispar and †Vegavis iaii [3,6,7,23].On a larger scale, the provenance of YPM VP 59473 underscores the ambiguity surrounding the geographic origins of living bird diversity.The earliest-diverging clades of living birds, such as the paleognaths, megapodes, magpie goose Anseranas, and anhimids, are all found in the southern hemisphere, a pattern that appears to support to an origin for crown birds in the southern continents [49].However, growing evidence from the fossil record has shown that these now exclusively southern hemisphere bird clades were once also distributed across the northern continents [10,19,[50][51][52][53]; the current distributions of clades as varied as mousebirds, seriemas, and hoatzins appear to represent contractions of far wider prehistoric ranges [54] The presence of YPM VP 59473 in North America supports a modified view of early crown avian biogeography that featured early cosmopolitanism followed by recent radiations into and throughout the southern hemisphere.
The discovery of YPM VP 59473 also provides new information on the ecological context of early crown bird evolution and survival through the Cretaceous-Paleogene (K-Pg) mass extinction, which killed all non-avian dinosaurs and all major stem bird clades.The ecologies of living birds and early members of the crown indicate that the K-Pg extinction globally selected against arboreal and large-bodied species [3,14,55].The Lance Formation ecosystem that YPM VP 59473 inhabited included a diverse assemblage of toothed stem-birds from at least four major clades (Fig. 7; [4]).A survey of the Lance Formation theropod fauna in the Yale Peabody Museum collections also shows that this unit has produced material assignable to eudromaeosaurian, alvarezsaurid, troodontid, and potentially 'four-winged' microraptorine dinosaurs (Supplementary Information).A serrated tooth resembling those of dromaeosaurids and small tyrannosaurids (e.g., [56,57]) is included in YPM VP 59473 and apparently came from the same block of conglomerate.Although the formation that produced European crown bird †Asteriornis maastrichtensis has also yielded a handful of non-avian dinosaur fossils, these are isolated and fragmentary 'bloat and float' bones that were washed out to sea and scavenged [58], and therefore do not necessarily come from the same ecosystem.The preservation of a well-preserved theropod tooth in the same block as YPM VP 59473, as well as the diversity of small-bodied nonavian theropod material recovered from various horizons in the Lance Formation, provides strong evidence for the coexistence of crown birds and non-avian dinosaurs in the northern hemisphere.
The coexistence of YPM VP 59473 and a diverse fauna of bird-like theropods and stem-birds (Fig. 7) raises the question of what factors might have mediated crown bird survival across the Cretaceous-Paleogene boundary, particularly as many features thought to have aided the survival of crown birds, such as a littoral ecology [2,44], arboreality and flight capacity [3,[59][60][61], dietary diversity [62][63][64][65], and terrestriality [3] are now known to have occurred across most of the major stem clades, including enantiornithines and ichthyornithines, that existed up to the time of the extinction.In sum, the anatomical and ecological information provided by YPM VP 59473 suggests that the survival of all three main clades of crown birds across the boundary (Fig. 7) is an excellent example of contingency in evolution.Rather than possessing a unique set of traits that facilitated their survival, crown birds may have been the 'happy few' that persisted across the K-Pg boundary as the more speciose and ecologically diverse stem-birds and non-avian dinosaurs went extinct around them.

Microscopy and measurements
Detailed characterization of the anatomy of bones and bone surfaces free of matrix was carried out using a standard light microscope at the Yale Peabody Museum.Ontogenetically informative characteristics were identified following several papers, especially [20][21][22] as well as via comparisons with juvenile bird skeletons at the YPM.
Measurements of the specimen were taken using digital calipers and are included in Table S1.

Computed tomography scanning and segmentation
Computed tomography (CT) scanning has facilitated the examination of three-dimensionally preserved Cretaceous avian fossils in exceptional detail without disturbing them from the matrix [2,34,40,55].The possibility that the conglomerate blocks included in YPM VP 59473 might store extensive avian material was considered following the description of †Asteriornis maastrichtensis and †Janavis finalidens from the late Maastrichtian of Belgium; both of these were recovered from block specimens [2,40].Highresolution CT scanning of YPM VP 59473 was conducted by M. Fox at the Yale Peabody Museum CT scanning facility.Scanning parameters are in Table S3.Individual elements were visualized and segmented using the programs 3D Slicer [66] and VGStudio MAX 3.5.Final renders were produced in the program Blender v 3.4.0.

Lance formation faunal survey
The collections of the Yale Peabody Museum contain numerous small vertebrate fossils from the 1903 J.B. Hatcher expedition to Niobrara County, Wyoming, and subsequent collection trips to Lance Formation outcrop.To understand the ecological community of YPM VP 59473, I conducted a survey of all catalogued fossil avian and nonavian theropod material in the Yale Peabody Museum, with Supplemental survey information from previous publications (e.g., [4]).Comparative anatomical work was Fig. 7 The ecological and temporal origins of living birds.Left side of the diagram shows the temporal and spatial range extensions and records of key small-bodied non-avian theropod clades found in the Lance Formation assemblage, and cladogram at right shows the major clades of stem and crown birds that survive to or past the K-Pg extinction, with ecologically relevant features that have been considered important to differential avian survival through that event noted along branches.All clades shown on tree are unambiguously represented in the Lance Formation assemblage, except Neoaves and Paleognathae.Divergence times for major lineages are primarily based on [2,13,14].Outlines are public domain from phylopic.orgor by the author.Bird illustrations are public domain by John Gould conducted using anatomical data collected from the Yale Peabody Museum, American Museum of Natural History, Muséum National D'histoire Naturelle, Denver Museum of Nature and Science, and New Jersey State Museum.Pie charts and other visualizations were made using the R 4.0.2 program ggplot2 3.4.2.The Supplementary Text contains additional description and assignment justifications for theropod fossils that are newly described in this contribution, which include the first probable records of microraptorines and alvarezsaurids of the genus †Trierarchuncus prairiensis [67] from the Lance Formation.

Fig. 1 Fig. 2
Fig. 1 Preservation of YPM VP 59473.The blocks containing all bones of the holotype (except for the humerus, tibiotarsus, synsacrum fragment, and large distal pedal phalanx) are shown under light microscopy (a, e, h) and with multiple x-ray views of the largest (b, c, d), second largest (f, g), and smallest (i) blocks as rendered in VGStudio, showing the relative placement of bones in the matrix blocks

Fig. 5
Fig. 5 Ontogenetic status of YPM VP 59473.Light microscopy of freed and in situ bones shows clear striated patterning across unfinished bone surfaces identical to bones of ontogenetically immature birds from the Recent