Fig. 2

MDA enhanced the migration of MCF7. The migration of MCF7 was assessed in the presence of the CM collected from MCF7 (MCF7_CM) or MDA (MDA_CM)—using a wound healing assays for 48 h or b transwell assays for 72 h, as well as c in monoculture (MC) and co-culture (CC) with MDA for 72 h—using wound-healing assays. MDA migration (using transwell assays) was also assessed after 24 h. d in the presence of MCF7_CM or e in co-culture with MCF7. Y-axis indicates the percentage of the occupied area (a, c, d and e) or the number of cells per field b. Representative images of the gap at the end of the wound healing assays (a, c) and of the migrated cells (dark purple patches) through the transwell inserts b are also shown. TGF-β1 (10 ng/ml) was used as a positive control. Error bars indicate SEM (n = 3). + signs denote differences relative to control (CT), and * signs refer to differences between treatments. ns = not significant; +/*, ++/**, +++/*** denote p < 0.05, p < 0.01, and p < 0.001, respectively