Fig. 2

Social Behaviors in a Resident/Intruder Assay Under Light/Dark Conditions. (A-B) Representative merged resident/intruder activity plots for surface fish (top) and Pachón cavefish (bottom) in the light (A) or dark (B) during resident/intruder interactions. (C-D) Quantifications of behaviors annotated during each assay with light (L) versus dark (D) intra-population comparisons for surface fish (C) and Pachón cavefish (D) Assays were performed in the light (surface fish, n = 10; Pachón cavefish, n = 10) and dark (surface fish, n = 9; Pachón cavefish, n = 10). Non-parametric Mann-Whitney tests were performed for all behaviors except for circling (both in C and D), for which an unpaired t-test was performed. Surface fish: biting (p = 0.6461), striking (0.5091), following (p = 0.9682), circling (p < 0.05), escaping (p = 0.6083), freezing (p = 0.1540), avoidance (p = 0.1121); Pachón cavefish: biting (p < 0.05), striking (p = 0.6979), following (p < 0.05), circling (p < 0.05), escaping (p = 0.9765), freezing (p > 0.9999), avoidance (p > 0.9999).p < 0.05 (*), p < 0.01 (**), p < 0.001 (***), p < 0.0001 (****), not significant (ns)