Fig. 1

Quantification of social behaviors in the resident/intruder assay for surface fish and Pachón cavefish. (A) Representative ethograms for pairs of surface fish (top) and Pachón cavefish (bottom) during the resident/intruder 1-hour assay. Seven behaviors were annotated: biting, striking, following, circling, escaping, freezing, and avoidance (Table 1) over the 60 min assay period. Behaviors were quantified for each fish, and were pooled for both fish in each resident/intruder assay here (surface: n = 10, Pachón: n = 11). (B-H) Quantifications of behaviors annotated during the resident/intruder assay. All behaviors were scored for both individuals in the tank, and each data point represents either the number of behavioral events (biting (B), striking (C), circling (E)) or the time spent in a behavioral state (following (D), escaping (F), freezing (G), avoidance (H)) for one trial. Unpaired t-tests were calculated for biting (p < 0.05), circling (p < 0.01) and freezing (p < 0.001). Mann-Whitney statistical tests were performed for striking (p < 0.0001), following (p < 0.05), escaping (p < 0.0001) and avoidance (p < 0.01). Significance: p < 0.05 (*), p < 0.01 (**), p < 0.001 (***), p < 0.0001 (****), not significant (ns)