Fig. 4

Distribution of predicted interacting residues on the D. melanogaster Regucalcin AAN09306.2 (A) and Dca AGB95961.1 (B) models. The overall predicted structures are highlighted in cyan, while the interacting residues are marked in yellow (one predicted interaction), orange (two predicted interactions) and red (three predicted interactions). The previously inferred PSS positions are represented by the corresponding amino acid symbol and sequence position in both proteins. The I¹⁴⁵,G¹⁷⁰ and S²¹⁰ Regucalcin positions (homologous to the Dca S¹³²,S¹⁵⁷ and S¹⁹⁷) are also displayed, as well as the homolog amino acids relative to the remaining datasets PSSs, with a “*” prefix. The Dca PSSs that were not correlated with the interaction regions (S¹⁵⁷ and S¹⁹⁷), as well as their homologues in Regucalcin (G¹⁷⁰ and S²¹⁰), are highlighted in magenta, while the remaining datasets PSSs homologs are shown in green. Although the distribution of interacting surfaces is distinct, the lid and active site regions of Regucalcin and Dca appear to be conserved