Table 2 Primers used for the qPCR
Target | Primer name | Sequence (5’-3’ direction) | Annealing temperature | Product size (bp) |
|---|---|---|---|---|
Haemoproteus | Cytb734F | ATGCAATTGTAGTAGATAGATATGT | 56 | 124 |
Cytb857R | GCTATCATAACTAATAAACCAGC | |||
Plasmodium [44] | PQF | CATGGATTTGTGGTGGATATCTTG | 52 | 198 |
PQR | TATCRAGACTTAAWAGATTTGGATAGAAG | |||
Host [43] | SFSR/3Fb | ACTAGCCCTTTCAGCGTCATGT | 57 | 114 |
SFSR/3Rb | CATGCTCGGGAACCAAAGG |