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Table 1 Detection of E. irwini eDNA in water samples collected along the Burdekin, Bowen and Broken River catchments

From: Environmental DNA analysis confirms extant populations of the cryptic Irwin’s turtle within its historical range

Catchment Site Biological replicates Technical replicates
# replicates # positive replicates % positive replicates # replicates # positive replicates % positive replicates
Burdekin The Rocksa [1] 5 1 20 40 1 2.5
Bogie Riverb [2] 5 0 0 40 0 0
Millarooa [3] 5 4 80 40 6 15
Expedition Pass Creekb [4] 5 0 0 40 0 0
Dalbega [5] 5 1 20 40 2 5
Johnny Cake Roada [6] 5 0 0 40 0 0
Burdekin and Bowen junctiona [7] 5 1 20 40 7 17.5
Blue Valley 1a [8] 5 0 0 40 0 0
Blue Valley 2a [9] 5 0 0 40 0 0
Gorge Weir 1a,c [10] 5 1 20 40 1 2.5
Gorge Weir 2a,c,* (11) 4 1 25 32 1 3.13
Burdekin Falls Dam downstream 1a [12] 5 4 80 40 12 30
Burdekin Falls Dam downstream 2a [13] 5 4 80 40 5 12.5
Bowen Terrible Creeka [14] 5 0 0 40 0 0
  Terrible Creek upstreama [15] 5 0 0 40 0 0
Riverviewa [16] 5 0 0 40 0 0
Bowen River Hotela [17] 5 0 0 40 0 0
Bowen River Hotel upstreama [18] 5 0 0 40 0 0
Pelican Creekb [19] 5 0 0 40 0 0
Myuna 1a [20] 5 0 0 40 0 0
Myuna 2a [21] 5 0 0 40 0 0
Birraleea [22] 5 2 40 40 4 10
Bowen Developmental Roada [23] 5 4 80 40 8 20
Exmoor Roada [24] 5 3 60 40 3 7.5
Broken Broken Rivera [25] 5 4 80 40 9 22.5
Mount Sugarloafa [26] 5 1 20 40 1 2.5
Grant Creekb [27] 5 0 0 40 0 0
Urannah Creek 1b [28] 5 2 40 40 7 17.5
Urannah Creek 2b [29] 5 5 100 40 27 67.5
Urannah Creek 3b [30] 5 4 80 40 7 17.5
Urannah Creek 4b [31] 5 3 60 40 19 47.5
Urannah Creek 5b [32] 5 5 100 40 19 47.5
Massey Gorgeb [33] 5 0 0 40 0 0
Blenheimb [34] 5 0 0 40 0 0
Old Racecourseb [35] 5 0 0 40 0 0
Resorta [36] 5 0 0 40 0 0
Bee Creekb [37] 5 0 0 40 0 0
  1. Numbers in brackets next to site name indicate the site number in Fig. 1. Sites with positive detections are indicated in bold
  2. aSites located on the main river channel; bsites located on a tributary; csites located on a weir; *one sample jar was compromised during transport