Table 1 Detection of E. irwini eDNA in water samples collected along the Burdekin, Bowen and Broken River catchments
Catchment | Site | Biological replicates | Technical replicates | ||||
|---|---|---|---|---|---|---|---|
# replicates | # positive replicates | % positive replicates | # replicates | # positive replicates | % positive replicates | ||
Burdekin | The Rocksa [1] | 5 | 1 | 20 | 40 | 1 | 2.5 |
Bogie Riverb [2] | 5 | 0 | 0 | 40 | 0 | 0 | |
Millarooa [3] | 5 | 4 | 80 | 40 | 6 | 15 | |
Expedition Pass Creekb [4] | 5 | 0 | 0 | 40 | 0 | 0 | |
Dalbega [5] | 5 | 1 | 20 | 40 | 2 | 5 | |
Johnny Cake Roada [6] | 5 | 0 | 0 | 40 | 0 | 0 | |
Burdekin and Bowen junctiona [7] | 5 | 1 | 20 | 40 | 7 | 17.5 | |
Blue Valley 1a [8] | 5 | 0 | 0 | 40 | 0 | 0 | |
Blue Valley 2a [9] | 5 | 0 | 0 | 40 | 0 | 0 | |
Gorge Weir 1a,c [10] | 5 | 1 | 20 | 40 | 1 | 2.5 | |
Gorge Weir 2a,c,* (11) | 4 | 1 | 25 | 32 | 1 | 3.13 | |
Burdekin Falls Dam downstream 1a [12] | 5 | 4 | 80 | 40 | 12 | 30 | |
Burdekin Falls Dam downstream 2a [13] | 5 | 4 | 80 | 40 | 5 | 12.5 | |
Bowen | Terrible Creeka [14] | 5 | 0 | 0 | 40 | 0 | 0 |
Terrible Creek upstreama [15] | 5 | 0 | 0 | 40 | 0 | 0 | |
Riverviewa [16] | 5 | 0 | 0 | 40 | 0 | 0 | |
Bowen River Hotela [17] | 5 | 0 | 0 | 40 | 0 | 0 | |
Bowen River Hotel upstreama [18] | 5 | 0 | 0 | 40 | 0 | 0 | |
Pelican Creekb [19] | 5 | 0 | 0 | 40 | 0 | 0 | |
Myuna 1a [20] | 5 | 0 | 0 | 40 | 0 | 0 | |
Myuna 2a [21] | 5 | 0 | 0 | 40 | 0 | 0 | |
Birraleea [22] | 5 | 2 | 40 | 40 | 4 | 10 | |
Bowen Developmental Roada [23] | 5 | 4 | 80 | 40 | 8 | 20 | |
Exmoor Roada [24] | 5 | 3 | 60 | 40 | 3 | 7.5 | |
Broken | Broken Rivera [25] | 5 | 4 | 80 | 40 | 9 | 22.5 |
Mount Sugarloafa [26] | 5 | 1 | 20 | 40 | 1 | 2.5 | |
Grant Creekb [27] | 5 | 0 | 0 | 40 | 0 | 0 | |
Urannah Creek 1b [28] | 5 | 2 | 40 | 40 | 7 | 17.5 | |
Urannah Creek 2b [29] | 5 | 5 | 100 | 40 | 27 | 67.5 | |
Urannah Creek 3b [30] | 5 | 4 | 80 | 40 | 7 | 17.5 | |
Urannah Creek 4b [31] | 5 | 3 | 60 | 40 | 19 | 47.5 | |
Urannah Creek 5b [32] | 5 | 5 | 100 | 40 | 19 | 47.5 | |
Massey Gorgeb [33] | 5 | 0 | 0 | 40 | 0 | 0 | |
Blenheimb [34] | 5 | 0 | 0 | 40 | 0 | 0 | |
Old Racecourseb [35] | 5 | 0 | 0 | 40 | 0 | 0 | |
Resorta [36] | 5 | 0 | 0 | 40 | 0 | 0 | |
Bee Creekb [37] | 5 | 0 | 0 | 40 | 0 | 0 | |