Environmental DNA analysis confirms extant populations of the cryptic Irwin’s turtle within its historical range

Table 1 Detection of E. irwini eDNA in water samples collected along the Burdekin, Bowen and Broken River catchments

Catchment	Site	Biological replicates			Technical replicates
Catchment	Site	# replicates	# positive replicates	% positive replicates	# replicates	# positive replicates	% positive replicates
Burdekin	The Rocks^a [1]	5	1	20	40	1	2.5
	Bogie River^b [2]	5	0	0	40	0	0
	Millaroo^a [3]	5	4	80	40	6	15
	Expedition Pass Creek^b [4]	5	0	0	40	0	0
	Dalbeg^a [5]	5	1	20	40	2	5
	Johnny Cake Road^a [6]	5	0	0	40	0	0
	Burdekin and Bowen junction^a [7]	5	1	20	40	7	17.5
	Blue Valley 1^a [8]	5	0	0	40	0	0
	Blue Valley 2^a [9]	5	0	0	40	0	0
	Gorge Weir 1^a,c [10]	5	1	20	40	1	2.5
	Gorge Weir 2^a,c,* (11)	4	1	25	32	1	3.13
	Burdekin Falls Dam downstream 1^a [12]	5	4	80	40	12	30
	Burdekin Falls Dam downstream 2^a [13]	5	4	80	40	5	12.5
Bowen	Terrible Creek^a [14]	5	0	0	40	0	0
	Terrible Creek upstream^a [15]	5	0	0	40	0	0
	Riverview^a [16]	5	0	0	40	0	0
	Bowen River Hotel^a [17]	5	0	0	40	0	0
	Bowen River Hotel upstream^a [18]	5	0	0	40	0	0
	Pelican Creek^b [19]	5	0	0	40	0	0
	Myuna 1^a [20]	5	0	0	40	0	0
	Myuna 2^a [21]	5	0	0	40	0	0
	Birralee^a [22]	5	2	40	40	4	10
	Bowen Developmental Road^a [23]	5	4	80	40	8	20
	Exmoor Road^a [24]	5	3	60	40	3	7.5
Broken	Broken River^a [25]	5	4	80	40	9	22.5
	Mount Sugarloaf^a [26]	5	1	20	40	1	2.5
	Grant Creek^b [27]	5	0	0	40	0	0
	Urannah Creek 1^b [28]	5	2	40	40	7	17.5
	Urannah Creek 2^b [29]	5	5	100	40	27	67.5
	Urannah Creek 3^b [30]	5	4	80	40	7	17.5
	Urannah Creek 4^b [31]	5	3	60	40	19	47.5
	Urannah Creek 5^b [32]	5	5	100	40	19	47.5
	Massey Gorge^b [33]	5	0	0	40	0	0
	Blenheim^b [34]	5	0	0	40	0	0
	Old Racecourse^b [35]	5	0	0	40	0	0
	Resort^a [36]	5	0	0	40	0	0
	Bee Creek^b [37]	5	0	0	40	0	0

Numbers in brackets next to site name indicate the site number in Fig. 1. Sites with positive detections are indicated in bold
^aSites located on the main river channel; ^bsites located on a tributary; ^csites located on a weir; *one sample jar was compromised during transport

ISSN: 2730-7182