Table 1 Primers used
From: Remnants of horizontal transfers of Wolbachia genes in a Wolbachia-free woodwasp
Primer | Target gene | Primer sequence (5′–3′) | References |
|---|---|---|---|
Wspecf | 16S | CATACCTATTCGAAGGGATAG | Werren and Windsor 2000 |
Wspecr | 16S | AGCTTCGAGTGAAACCAATTC | Werren and Windsor 2000 |
pA (27 F) | 16S | AGAGTTTGATCMTGGCTCAG | Edwards et al. 1989 |
EHR 16SR | 16S | GTAATCGTGGATCATCATGC | Parola et al. 2000 |
EHR 16SD | 16S | GGTACCYACAGAAGAAGTCC | Parola et al. 2000 |
pH (1492 R) | 16S | TACGGYTACCTTGTTACGACTT | Reysenbach et al. 1992 |
16S 567F | 16S | ATYATTGGGCGTAAAGGG | This study |
16S 712F | 16S | TATTAGGAGGAACACCRGT | This study |
16S 712R | 16S | ACYGGTGTTCCTCCTAATA | This study |
16S 1401R | 16S | AGTGTGTACAAGACCCGAG | This study |
Wsp 81 F | wsp | TGGTCCAATAAGTGATGAAGAAAC | Braig et al. 1998 |
Wsp 691 R | wsp | AAAAATTAAACGCTACTCCA | Braig et al. 1998 |
ftsZf1 | FtsZ | GTTGTCGCAAATACCGATGC | Werren et al. 1995 |
ftsZr1 | FtsZ | CTTAAGTAAGCTGGTATATC | Werren et al. 1995 |
SnW1f | ORF4 | TACCGCCAAAGTGTTCATCA | This study |
SnW1r | ORF4 | TGCCATCTGGTGAAATTGAA | This study |
SnW2f | ORF5 | TCCATAAGTGGGCTCTCACC | This study |
SnW2r | ORF5 | AGAGCCGAACGCTTATATGG | This study |
SnW3f | ORF8 | CACACCTTCTGGAATGCTGA | This study |
SnW3r | ORF8 | AAAGTTGCGCTACCTGATGG | This study |