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Fig. 3 | BMC Ecology and Evolution

Fig. 3

From: Widely used, short 16S rRNA mitochondrial gene fragments yield poor and erratic results in phylogenetic estimation and species delimitation of amphibians

Fig. 3

An illustration depicting how the three datasets used in this study were generated. The Full dataset comprised of 147 full-length 16S sequences. The Medium and Short datasets are subsets of the Full alignment and thus, contain the same sequences. Supplementary reference sequences were used to determine the appropriate trimming sites, ensuring that subset alignments were trimmed according to established primer binding sites. Reference sequences were not included in the final datasets as they are not comparable with longer sequences

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