Fig. 4

eDNA released of collected eDNA samples at 3 time points for 72 h after addition of 0 mL, 15 mL, 30 mL, and 45 mL of crab stock solution to 15-L of sterile seawater. Mean ± SEM, error bars are shown in only one direction for some data points for clarity of the figure. N = 5 per data point. Data points are nudged slightly along the x-axis to avoid overlaying of the respective symbols. Though not visible due to scale, one qPCR replicate from the 0 mL treatment yielded a false positive amplification